Belokurova V B, Golovach I S, Shcherbak N L, Kuchuk N V
Tsitol Genet. 2004 May-Jun;38(3):9-15.
Methods of in vitro cultivation of an African endemic species Nicotiana africana (Solanaceae) possessing some valuable traits have been elaborated. Influence of different concentrations of auxin (alpha-naphtaleneacetic acid) and cytokinins (6-benzylaminopurine, zeatin, thidiazuron) on morphogenesis and plant regeneration has been analysed using leaves and internodes as explants. The optimum method of regeneration of N. africana shoots in leaf explants is cultivation in the presence of 0.1 mg/l NAA and 1 mg/l BA; in internode explants--cultivation on the medium containing 0.5 mg/l NAA and 1 mg/l zeatin. The use of thidiazuron was the most effective at the concentration of 0.4 mg/l with subsequent transfer of explants to hormone-free medium. Method of isolation and culture of mesophyll protoplasts enabling to regenerate N. africana plants during 3-4 months has been proposed.
已详细阐述了对具有一些有价值性状的非洲特有物种烟草（茄科）进行体外培养的方法。使用叶片和节间作为外植体，分析了不同浓度的生长素（α-萘乙酸）和细胞分裂素（6-苄基氨基嘌呤、玉米素、噻苯隆）对形态发生和植株再生的影响。烟草叶片外植体中芽再生的最佳方法是在含有0.1mg/l萘乙酸和1mg/l苄氨基腺嘌呤的条件下培养；节间外植体则是在含有0.5mg/l萘乙酸和1mg/l玉米素的培养基上培养。噻苯隆在浓度为0.4mg/l时效果最为显著，随后将外植体转移至无激素培养基上。已提出了分离和培养叶肉原生质体的方法，该方法能使烟草植株在3 - 4个月内再生。
