Chandramouli K H, Thimmaiah K N, Chandrashekar Arun, D'Souza Cletus J M
Department of Biochemistry, University of Mysore, Mysore, India.
Nucleosides Nucleotides Nucleic Acids. 2004;23(10):1639-56. doi: 10.1081/NCN-200031461.
Five N10-substituted phenoxazines having different R groups and -Cl substitution at C-2 were found to bind to calf -thymus DNA and plasmid DNA with high affinity as seen from by UV and CD spectroscopy. The effect of phenoxazines on DNA were studied using DNA-ethidium bromide complexes. Upon addition of phenoxazines, the ethidium bromide dissociated from the complex with DNA. The binding of phenoxazines to plasmid PUC18 reduced ethidium bromide binding as seen from the agarose gel electrophoresis. Butyl, and propyl substituted phenoxazines were able to release more ethidium bromide compared with that of acetyl substitution. Addition of phenoxazines also enhanced melting temperature of DNA.
通过紫外光谱和圆二色光谱发现，五种在N10位具有不同R基团且在C-2位有-Cl取代的吩恶嗪能以高亲和力与小牛胸腺DNA和质粒DNA结合。使用DNA-溴化乙锭复合物研究了吩恶嗪对DNA的影响。加入吩恶嗪后，溴化乙锭从与DNA的复合物中解离。从琼脂糖凝胶电泳可以看出，吩恶嗪与质粒PUC18的结合降低了溴化乙锭的结合。与乙酰取代的吩恶嗪相比，丁基和丙基取代的吩恶嗪能够释放更多的溴化乙锭。加入吩恶嗪还提高了DNA的解链温度。
