Liu De-yu, Zheng Hong-qiang, Luo Gao-qing
Chemistry Department, Medical Sciences College, Sun Yat-sen University, Guangzhou 510089, Guangdong, China.
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):957-61.
To investigate the effects of ampelopsin on B16 melanoma's invasion and metastasis in vivo and in vitro.
B16 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form an experimental pulmonary metastasis of tumor cell. The ampelopsin was administered at 3 dosages by intraperitoneal injection daily for 18 days from the day before the cells injection. The B16 mouse melanoma cells were exposed to ampelopsin for 3 days. The effects of ampelopsin on invasion, migration and adhesion of B16 melanoma cells were evaluated with Transwell chambers or attachment with polycarbonate filters and reconstituted basement membrane (Matrigel).
The number of metastases in the animals that were given ampelopsin 150, 200, and 250 mg x kg(-1) x d(-1) was significantly reduced as compared to the vehicle control (P<0.05), and the inhibition rates were 30.97%, 40.58%, and 61.16%, respectively. The ability of the ampelopsin treated B16 cells to invade the reconstituted basement membrane was decreased significantly (P<0.01), and the inhibition rates were 36.06%, 59.58%, and 79.09% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1) concentration, respectively. Ampelopsin could also inhibit B16 cells migration through PVPF in the Transwell chambers, and the inhibition rates were 51.59%, 56.51%, and 66.75% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1), respectively (P<0.01). The ability of adhesion of the B16 cells by ampelopsin treated cells on fibronectin, laminin, or Matrigel was decreased significantly.
Ampelopsin has anti-invasive and anti-metastatic effects on B16 melanoma.
研究白藜芦醇对B16黑色素瘤体内外侵袭和转移的影响。
将B16小鼠黑色素瘤细胞经尾侧静脉注射到C57BL/6小鼠体内，随后在动物肺部定植形成肿瘤细胞的实验性肺转移。从注射细胞前一天开始，每天腹腔注射3种剂量的白藜芦醇，持续18天。将B16小鼠黑色素瘤细胞暴露于白藜芦醇中3天。用Transwell小室或与聚碳酸酯滤膜和重组基底膜（基质胶）附着的方法评估白藜芦醇对B16黑色素瘤细胞侵袭、迁移和黏附的影响。
给予150、200和250mg·kg⁻¹·d⁻¹白藜芦醇的动物转移灶数量与溶媒对照组相比显著减少（P<0.05），抑制率分别为30.97%、40.58%和61.16%。白藜芦醇处理的B16细胞侵袭重组基底膜的能力显著降低（P<0.01），在20μmol·L⁻¹、40μmol·L⁻¹和80μmol·L⁻¹浓度下的抑制率分别为36.06%、59.58%和79.09%。白藜芦醇还可抑制B16细胞在Transwell小室中通过聚碳酸酯滤膜的迁移，在20μmol·L⁻¹、40μmol·L⁻¹和80μmol·L⁻¹时的抑制率分别为51.59%、56.51%和66.75%（P<0.01）。白藜芦醇处理的细胞对B16细胞在纤连蛋白、层粘连蛋白或基质胶上的黏附能力显著降低。
白藜芦醇对B16黑色素瘤具有抗侵袭和抗转移作用。
