Rodrigues Jairo Lisboa, de Magalhães Cristiana Schmidt, Luccas Pedro Orival
Departamento de Ciências Exatas, Escola de Farmácia e Odontologia de Alfenas-Centro Universitário Federal, Cep 37130-000 Alfenas, MG, Brazil.
J Pharm Biomed Anal. 2005 Jan 4;36(5):1119-23. doi: 10.1016/j.jpba.2004.09.016.
A flow analysis (FA) system with spectrophotometric detection for Al determination in hemodialysis solutions was developed. The method was based on the reaction of Al with eriochrome cyanine R (ECR). The complex formed associated with cetyltrimethylammonium bromide (CTAB)--a cationic surfactant, which showed enough sensitivity to execute the direct analyte determination. All interferences were eliminated with the matrix matching calibration. The system presented the following analytic parameters: sensitivity (m) of 8.10 x 10(-4)L microg(-1), limit of detection (LOD) of 3.24 microg L(-1) (3sigma), linear correlation coefficient of 0.9966 and linear range response from 10.8 to 650 microg L(-1). The accuracy of the proposed method was checked by comparison with electrothermal atomic absorption spectrometry (ET-AAS) method. There were no differences among the results obtained from both methods, at a confidence level of 95% (paired t-test). Recovery tests were also made, values obtained were from 90.4 to 109 of recovery for Al-spiked samples.
开发了一种用于测定血液透析溶液中铝的带分光光度检测的流动分析（FA）系统。该方法基于铝与铬菁R（ECR）的反应。形成的络合物与阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）缔合，其显示出足够的灵敏度以进行直接分析物测定。通过基体匹配校准消除了所有干扰。该系统呈现以下分析参数：灵敏度（m）为8.10×10⁻⁴ L μg⁻¹，检测限（LOD）为3.24 μg L⁻¹（3σ），线性相关系数为0.9966，线性范围响应为10.8至650 μg L⁻¹。通过与电热原子吸收光谱法（ET-AAS）比较来检验所提出方法的准确性。在95%的置信水平下（配对t检验），两种方法获得的结果之间没有差异。还进行了回收率测试，加标铝样品的回收率值为90.4%至109%。
