Pavlok A, Lucas-Hahn A, Niemann H
Institut für Tierzucht und Tierverhalten (FAL), Mariensee, Neustadt, Federal Republic of Germany.
Mol Reprod Dev. 1992 Jan;31(1):63-7. doi: 10.1002/mrd.1080310111.
This study investigated the capacity of healthy oocytes derived from follicles of different size to undergo normal fertilization and early embryonic development in vitro and full-term development in vivo. Ovaries were collected from a local abattoir and dissected and classified as follows: group A, greater than 4-8 mm (large); group B, greater than 2-4 mm (medium); and group C, greater than 1-2 mm (small). Oocytes were isolated by puncturing the follicular wall and pressing of the follicle. Only healthy-looking cumulus-oocyte complexes (COC) were used for in vitro maturation. Oocytes were fertilized in vitro by frozen/thawed semen from one bull. Approximately one-fourth of all oocytes was fixed and stained 15-20 h after fertilization, to determine penetration rates. The remaining eggs were transferred to culture medium and were cultivated for up to 9.5 days. Cleavage was observed 65 h and 7 days after fertilization. Expanded, hatching, and hatched blastocysts were fixed and stained after 9.5 days of culture. A total of 86 blastocysts derived from group A and B oocytes was nonsurgically transferred to synchronized recipients 7-8 days after onset of culture. A total of 6.624 follicles were dissected from 265 ovaries, and 1,485 oocytes were isolated from 1,671 group A follicles, 3,509 oocytes from 3,862 group B follicles, and 965 oocytes from 1,091 group C follicles. The fertilization rate, rate of normal fertilization, rate of polyspermy, and rate of other abnormal fertilization features were as follows: group A, 84.9%, 43.2%, 34.1%, 7.6%; group B, 83.6%, 44.8%, 31.1%, 7.8%; and group C, 61.7%, 13.1%, 33.7%, 19.1%.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究调查了源自不同大小卵泡的健康卵母细胞在体外进行正常受精和早期胚胎发育以及在体内进行足月发育的能力。从当地屠宰场收集卵巢并进行解剖，分类如下：A组，大于4 - 8毫米（大）；B组，大于2 - 4毫米（中）；C组，大于1 - 2毫米（小）。通过穿刺卵泡壁并挤压卵泡来分离卵母细胞。仅使用外观健康的卵丘 - 卵母细胞复合体（COC）进行体外成熟。卵母细胞通过一头公牛的冷冻/解冻精液进行体外受精。受精后15 - 20小时，将所有卵母细胞的约四分之一固定并染色，以确定受精率。其余的卵被转移到培养基中，培养长达9.5天。在受精后65小时和7天观察到卵裂。培养9.5天后，将扩张、孵化和已孵化的囊胚固定并染色。在培养开始7 - 8天后，将总共86个源自A组和B组卵母细胞的囊胚非手术转移到同步化的受体中。从265个卵巢中总共解剖出6624个卵泡，从1671个A组卵泡中分离出1485个卵母细胞，从3862个B组卵泡中分离出3509个卵母细胞，从1091个C组卵泡中分离出965个卵母细胞。受精率、正常受精率、多精受精率和其他异常受精特征率如下：A组，84.9%、43.2%、34.1%、7.6%；B组，83.6%、44.8%、31.1%、7.8%；C组，61.7%、13.1%、33.7%、19.1%。（摘要截断于250字）
