Barnard K, Burgess S A, Carter D A, Woolley D M
Comparative Pathology Laboratory, School of Veterinary Science, University of Bristol, Langford, United Kingdom.
J Struct Biol. 1992 Jan-Feb;108(1):6-13. doi: 10.1016/1047-8477(92)90002-r.
The anterior lens capsule provides a thick, easily handled model system for the study of the organization of type IV collagen, the main component of basement membranes. We have used the technique of rapid freezing, deep-etch, and rotary replication to study the three-dimensional organization of the collagen skeleton in mammalian lens capsule after a variety of extraction procedures. In all cases the collagen appeared as a densely packed three-dimensional branching network of fine microfibrils. The organization of the microfibrils appears to show some regularity, with branch points approximately 40 nm apart. Most junctions are three-way and the network forms predominantly five-sided figures. This closely resembles the collagenous network described by Yurchenco and Ruben (1987, 1988) in human amniotic basement membrane and EHS tumor matrix, but extends their findings to another system for which X-ray diffraction data are available. The three-dimensional network is discussed in terms of molecular packing of type IV collagen in light of the information available from the diffraction data.
晶状体前囊膜为研究IV型胶原（基底膜的主要成分）的组织结构提供了一个厚实、易于操作的模型系统。我们运用快速冷冻、深度蚀刻和旋转复型技术，研究了经过各种提取程序后的哺乳动物晶状体囊膜中胶原骨架的三维组织结构。在所有情况下，胶原均呈现为紧密堆积的由细微原纤维构成的三维分支网络。原纤维的组织结构似乎呈现出一定的规律性，分支点间距约为40纳米。大多数连接点为三叉，网络主要形成五边形结构。这与Yurchenco和Ruben（1987年、1988年）在人羊膜基底膜和EHS肿瘤基质中描述的胶原网络极为相似，但将他们的研究结果扩展到了另一个可获取X射线衍射数据的系统。结合从衍射数据中获得的信息，对IV型胶原的分子堆积方式进行了三维网络的讨论。
