[白细胞介素-2和α-干扰素激活的骨髓对K562白血病细胞的杀伤作用]
[The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell].
作者信息
Li C, Yan W, Hao Y, Feng S, Qiu L, Liu H, Han J, Li X, Feng S
机构信息
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 1997 Aug;18(8):410-2.
OBJECTIVE
To evaluate the killing effect of IL-2 and IFN-alpha activated bone marrow cells on K562 cells.
METHODS
Semi-solid colony assay was used.
RESULTS
Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31 approximately 2.30 logs on K562 cells, this killing effects was further increased to 0. 30 approximately > 3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells.
CONCLUSION
IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM.
目的
评估白细胞介素-2（IL-2）和α干扰素（IFN-α）激活的骨髓细胞对K562细胞的杀伤作用。
方法
采用半固体集落分析法。
结果
白血病缓解期患者的骨髓在体外经IL-2激活3天。激活的骨髓（ABM）对K562细胞显示出0.31至约2.30对数的杀伤作用，当在骨髓激活时将IFN-α与IL-2一起添加，这种杀伤作用进一步增加至0.30至约>3.15对数。单独的IL-2或与IFN-α联合使用均未显示对CFU-GM和K562细胞有抑制作用。与单独使用IL-2或IFN-α相比，两种细胞因子联合使用能更有效地维持ABM对白血病细胞的杀伤作用。
结论
IFN-α可增强IL-2激活的ABM的净化作用，两种细胞因子联合使用能有效维持ABM的细胞毒性。
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