烟草1号蛋白小亚基序列异质性的起源
Origin of sequence heterogeneity of the small subunit of fraction 1 protein from Nicotiana tabacum.
作者信息
Iwai S, Tanabe Y, Kawashima N
机构信息
Central Research Institute, The Japan Tobacco Public Corporation, 6-2 Umegaoka, Midori-ku, Yokohama, Kanagawa 227, Japan.
出版信息
Biochem Biophys Res Commun. 1976 Dec 20;73(4):993-6. doi: 10.1016/0006-291x(76)90220-5.
The partial amino acid sequences of the small subunit of Fraction 1 protein from N. sylvestris, N. tomentosiformis and N. tabacum were determined. The sequence of N. sylvestris is NH2. Gln-Val-Trp-Pro-Pro-Ile-Asn-----Tyr COOH. In the sequence up to the 7th amino acid and C-terminus, differences were only found at the 6th position in the three species, where N. sylvestris and N. tomentosiformis show Ile and Tyr, respectively. N. tabacum show both Ile and Tyr in almost equal amount at this position. These results confirmed a previous hypothesis that N. tabacum had been evolved through the hybridization of N. sylvestris and N. tomentosiformis.
测定了野生烟草、绒毛状烟草和烟草中1号蛋白小亚基的部分氨基酸序列。野生烟草的序列为NH2。谷氨酰胺-缬氨酸-色氨酸-脯氨酸-脯氨酸-异亮氨酸-天冬酰胺-----酪氨酸COOH。在直至第7个氨基酸和C末端的序列中，仅在这三个物种的第6个位置发现差异，其中野生烟草和绒毛状烟草在该位置分别显示异亮氨酸和酪氨酸。烟草在该位置几乎等量地显示异亮氨酸和酪氨酸。这些结果证实了先前的一个假设，即烟草是通过野生烟草和绒毛状烟草的杂交进化而来的。
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