脯氨酰羟化酶及一种相关免疫反应性蛋白的体内标记与更新
In vivo labeling and turnover of prolyl hydroxylase and a related immunoreactive protein.
作者信息
Chichester C O, Fuller G C, Cardinale G J
机构信息
Department of Pharmacology and Toxicology, University of Rhode Island, Kingston, Rhode Island 02881, USA.
出版信息
Biochem Biophys Res Commun. 1976 Dec 20;73(4):1056-62. doi: 10.1016/0006-291x(76)90230-8.
Prolyl hydroxylase and an immunologically related protein (CRP) were purified from neonatal rabbit skin at various time periods following administration of 3H-leucine. The peak incorporation of label into prolyl hydroxylase was found to be 12 hours, while peak incorporation into CRP occurred within 2 hours. Semi-log plots of the loss of radioactivity from these protein pools against time indicated an apparent T 1/2 for prolyl hydroxylase of 78 hours, and a T 1/2 of CRP of 44 hours. Calculated Kd values indicate that that breakdown of active enzyme does not account for the amount of CRP found in tissues.
在给予³H-亮氨酸后的不同时间段，从新生兔皮肤中纯化出脯氨酰羟化酶和一种免疫相关蛋白（CRP）。发现标记物掺入脯氨酰羟化酶的峰值出现在12小时，而掺入CRP的峰值在2小时内出现。这些蛋白质库中放射性损失随时间的半对数图表明，脯氨酰羟化酶的表观半衰期为78小时，CRP的半衰期为44小时。计算出的解离常数（Kd）值表明，活性酶的分解并不能解释组织中发现的CRP量。
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