Chen-Kiang S, Cardinale G J, Udenfriend S
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4420-4. doi: 10.1073/pnas.74.10.4420.
A protein, enzymatically inactive but immunologically related to prolyl hydroxylase (prolyl-glycyl-peptide, 2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2) (cross-reacting protein), has been purified to near homogeneity from skin of newborn rats. The purified protein has a molecular weight of 60,000 on gel filtration and sodium dodecyl sulfate gel electrophoresis, corresponding to that of the smaller of the two dissimilar subunits of the enzyme. The two subunits of prolyl hydroxylase differ markedly from one another in their amino acid compositions, but crossreating protein and the smaller subunit are very similar in composition. On antibody-affinity chromatography both subunits reacted with the antibody developed against the intact enzyme. Neither crossreacting protein nor the 60,000 molecular weight subunit was adsorbed onto concanavalin A, which adsorbed the intact enzyme as well as the larger subunit. It would appear that crossreacting protein is identical to one of the subunits of prolyl hydroxylase or metabolically related to it.
一种蛋白质已从新生大鼠的皮肤中纯化至接近同质状态，该蛋白质无酶活性，但与脯氨酰羟化酶（脯氨酰 - 甘氨酰 - 肽，2 - 氧代戊二酸：氧氧化还原酶；EC 1.14.11.2）（交叉反应蛋白）具有免疫相关性。经凝胶过滤和十二烷基硫酸钠凝胶电泳测定，纯化后的蛋白质分子量为60,000，与该酶两个不同亚基中较小的亚基分子量相当。脯氨酰羟化酶的两个亚基在氨基酸组成上差异显著，但交叉反应蛋白与较小亚基的组成非常相似。在抗体亲和色谱上，两个亚基均与针对完整酶产生的抗体发生反应。交叉反应蛋白和分子量为60,000的亚基均未被伴刀豆球蛋白A吸附，而伴刀豆球蛋白A能吸附完整酶以及较大亚基。看来交叉反应蛋白与脯氨酰羟化酶的一个亚基相同或与其存在代谢关联。
