Filonenko Valeriy V, Tytarenko Ruslana, Azatjan Sergey K, Savinska Lilya O, Gaydar Yuriy A, Gout Ivan T, Usenko Vasiliy S, Lyzogubov Valeriy V
Institute of Molecular Biology and Genetic, NAS of Ukraine, Kyiv, Ukraine.
Exp Oncol. 2004 Dec;26(4):294-9.
To perform an immunohistochemical analysis of human breast adenomas and adenocarcinomas as well as normal breast tissues in respect of S6 ribosomal protein kinase (S6K) expression and localization in normal and transformed cells.
The expression level and localization of S6K have been detected in formalin fixed, paraffin embedded sections of normal human breast tissues, adenomas and adenocarcinomas with different grade of differentiation. Immunohistochemical detection of S6K1 and S6K2 in normal human breast tissues and breast tumors were performed using specific monoclonal and polyclonal antibodies against S6K1 and S6K2 with following semiquantitative analysis.
The increase of S6K content in the cytoplasm of epithelial cells in benign and malignant tumors has been detected. Nuclear accumulation of S6K1 and to a greater extend S6K2 have been found in breast adenocarcinomas. About 80% of breast adenocarcinomas cases revealed S6K2 nuclear staining comparing to normal tissues. In 31% of cases more then 50% of cancer cells had strong nuclear staining. Accumulation of S6K1 in the nucleus of neoplastic cells has been demonstrated in 25% of cases. Nuclear localization of S6K in the epithelial cells in normal breast tissues has not been detected.
Immunohistochemical analysis of S6K1 and S6K2 expression in normal human breast tissues, benign and malignant breast tumors clearly indicates that both kinases are overexpressed in breast tumors. Semiquantitative analysis of peculiarities of S6K localization in normal tissues and tumors revealed that nucleoplasmic accumulation of S6K (especially S6K2) is a distinguishing feature of cancer cells.
对人乳腺腺瘤、腺癌以及正常乳腺组织进行免疫组织化学分析，以研究S6核糖体蛋白激酶（S6K）在正常及转化细胞中的表达和定位情况。
在经福尔马林固定、石蜡包埋的不同分化程度的正常人乳腺组织、腺瘤及腺癌切片中检测S6K的表达水平和定位。使用针对S6K1和S6K2的特异性单克隆和多克隆抗体，对正常人乳腺组织和乳腺肿瘤进行S6K1和S6K2的免疫组织化学检测，并进行如下半定量分析。
已检测到良性和恶性肿瘤上皮细胞胞质中S6K含量增加。在乳腺腺癌中发现S6K1在细胞核中积聚，且S6K2在细胞核中的积聚更为明显。与正常组织相比，约80%的乳腺腺癌病例显示S6K2细胞核染色。在31%的病例中，超过50%的癌细胞有强细胞核染色。在25%的病例中证实肿瘤细胞的细胞核中有S6K1积聚。在正常乳腺组织的上皮细胞中未检测到S6K的细胞核定位。
对正常人乳腺组织、乳腺良性和恶性肿瘤中S6K1和S6K2表达的免疫组织化学分析清楚地表明，这两种激酶在乳腺肿瘤中均过度表达。对S6K在正常组织和肿瘤中定位特点的半定量分析显示，S6K（尤其是S6K2）在核质中的积聚是癌细胞的一个显著特征。
