Colin F C, Gallois Y, Rapin D, Meskar A, Chabaud J J, Vicariot M, Ménez J F
Laboratoire d'Hématologie, CHU Morvan, Brest, France.
Blood. 1992 Apr 15;79(8):2148-53.
The lipid composition of erythrocytes (red blood cells [RBCs]) plays a significant role in determining certain membrane biophysical properties. We have found that fetal RBCs showed a dramatically low filterability compared with adult RBCs and questioned whether this could be a consequence of their membrane lipid composition. We therefore studied fetal RBCs at two different gestational ages, neonatal RBCs and adult RBCs. Biophysical parameters were studied using two different techniques, filterability and membrane fluidity. The latter was measured by fluorescence polarization using three different probes. The membrane lipid composition was examined by measuring cholesterol and phospholipids. After extraction of the phospholipids, followed by high performance thin-layer chromatography, the fatty acids in the phospholipid subfractions were analyzed by gas-liquid chromatography. The fetal RBCs' filterability was found to be correlated with both the larger size and the higher hemoglobin content of the cells, but there was no correlation between RBC filterability and fluidity or membrane lipid composition. In adult RBCs, compared with neonatal RBCs, the slight increase of unsaturated fatty acids in phosphatidylcholine and phosphatidylethanolamine should have increased the membrane fluidity. However, in RBCs, no change was observed in the fluidity parameters measured by fluorescence polarization.
红细胞（红血球[RBC]）的脂质组成在决定某些膜生物物理特性方面起着重要作用。我们发现，与成人红细胞相比，胎儿红细胞的过滤性极低，于是质疑这是否可能是其膜脂质组成所致。因此，我们研究了两个不同胎龄的胎儿红细胞、新生儿红细胞和成人红细胞。使用两种不同技术研究了生物物理参数，即过滤性和膜流动性。后者通过使用三种不同探针的荧光偏振进行测量。通过测量胆固醇和磷脂来检查膜脂质组成。提取磷脂后，进行高效薄层色谱分析，然后通过气液色谱分析磷脂亚组分中的脂肪酸。发现胎儿红细胞的过滤性与细胞的较大尺寸和较高血红蛋白含量均相关，但红细胞过滤性与流动性或膜脂质组成之间没有相关性。在成人红细胞中，与新生儿红细胞相比，磷脂酰胆碱和磷脂酰乙醇胺中不饱和脂肪酸的轻微增加本应增加膜流动性。然而，在红细胞中，通过荧光偏振测量的流动性参数未观察到变化。
