Bidlingmeyer Brian A, Henderson John
Agilent Technologies, Little Falls Site, 2850 Centerville Road, Wilmington, DE 19808, USA.
J Chromatogr A. 2004 Dec 10;1060(1-2):187-93.
The use of unbonded silica as a stationary phase in reversed-phase HPLC is described as a useful alternative to bonded phase columns for polar, lipophilic amines. Using four lipophilic amines, the role of temperature is shown to favorably impact both efficiency and selectivity, which is not universally seen when using bonded phases. As temperature is raised, retention drops on the silica column. The temperature behavior appears to support the hypothesis that retention is dependant upon electrostatic and adsorptive forces.
在反相高效液相色谱中使用未键合硅胶作为固定相，被描述为对于极性亲脂性胺类而言，是键合相柱的一种有用替代物。使用四种亲脂性胺类时，温度的作用显示出对柱效和选择性均有有利影响，而在使用键合相时并非普遍如此。随着温度升高，硅胶柱上的保留时间下降。温度行为似乎支持保留取决于静电和吸附力这一假说。
