[土鳖虫对血瘀证大鼠红细胞CR1活性及抗心磷脂抗体水平的影响]

[Effects of Eupolyphaga Sinensis Walker on erythrocyte's CR1 activity and anti-cardiolipin antibody level in rats with stagnation of blood].

作者信息

Yang Yao-Fang, Wang Sai-Qian, Feng Mei-Jia, Ning Ai-Hua, Huang Chang-Hui

机构信息

Research Room of Pharmacology, Shanghai Fifth Peopleos Hospital Affiliated to Fudan University, Shanghai 200240, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Jan;21(1):53-6.

PMID:15629084

Abstract

AIM

To study the effect of Eupolyphaga Sinensis Walker(ESW) on red blood cell immune adherence (RCIA) and serum anticardiolipin antibody level in rat model of Yin-deficiency Huo-excess with chronic blood stasis.

METHODS

The model was made by i.m. injection of dexamethasone (0.5 mg/kg) and adrenaline (0.84 mg/kg). The serum anti-cardiolipin antibodies (ACA) ACA-IgG, ACA-IgA and ACA-IgM and the plasma D-dimmer levels were measured by using enzyme-linked immunosorbent assay (ELISA). The body and organ weight of the rats were measured.

RESULTS

For rats of Yin-deficiency Huo-excess, rosette rates of red blood cell C3b receptors (RBC-C3b RR) and red blood cell cancer (RBC-CaR) decreased, the serum ACA-IgG, ACA-IgA, ACA-IgM and the plasma D-dimmer levels markedly increased, body weight and the weight of spleen and thymus all decreased. ESW (5 mg/kg, 10 mg/kg) increased RBC-C3bRR and RBC-CaR, reduced serum ACA-IgG, ACA-IgA, ACA-IgM and plasma D-dimmer levels, and increased spleen weight of the rats.

CONCLUSION

ESW can boost the immune function in rats of Yin-deficiency Huo-excess with chronic blood stasis.

摘要

目的

研究中华地鳖虫对阴虚火旺兼慢性血瘀证大鼠模型红细胞免疫黏附（RCIA）及血清抗心磷脂抗体水平的影响。

方法

采用肌肉注射地塞米松（0.5mg/kg）和肾上腺素（0.84mg/kg）制备模型。采用酶联免疫吸附测定（ELISA）法检测血清抗心磷脂抗体（ACA）ACA-IgG、ACA-IgA和ACA-IgM以及血浆D-二聚体水平。测量大鼠的体重和器官重量。

结果

阴虚火旺大鼠红细胞C3b受体花环率（RBC-C3b RR）和红细胞癌花环率（RBC-CaR）降低，血清ACA-IgG、ACA-IgA、ACA-IgM及血浆D-二聚体水平明显升高，体重及脾脏和胸腺重量均降低。中华地鳖虫（5mg/kg、10mg/kg）可提高RBC-C3bRR和RBC-CaR，降低血清ACA-IgG、ACA-IgA、ACA-IgM及血浆D-二聚体水平，并增加大鼠脾脏重量。