吸烟者与非吸烟者之间氧化应激生物标志物“尿8-羟基-2'-脱氧鸟苷”的比较。

Comparison of an oxidative stress biomarker "urinary8-hydroxy-2'-deoxyguanosine," between smokers and non-smokers.

作者信息

Kanaya Setsuko, Ikeya Masae, Yamamoto Kanako, Moriya Takayo, Furuhashi Keiko, Sonoda Makiko, Goto Koichi, Ochi Hirotomo

机构信息

Department of Clinical Nutrition, Seirei Mikatabara Hospital, Japan.

出版信息

Biofactors. 2004;22(1-4):255-8. doi: 10.1002/biof.5520220151.

DOI:10.1002/biof.5520220151

PMID:15630293

Abstract

A great deal of effort has been made on the effect of oxidative stress for smokers. What seems to be lacking, however, is its evidence. Analyzing 1076 participants (age 35.9 +/- 12.9, urinary8-OHdG Mean +/- S.D., 11.4 +/- 6.7, n = 1076), our study found the significant increase in a biomarker of DNA damage urinary 8-OHdG/creatinine among smokers (7.75 +/- 2.8 ng/ml x CRE (n = 154) and 7.36 +/- 2.5 ng/ml x CRE (n = 627) (p < 0.05), Relative Risk = 2.9 (1.4-6.2) sex and age +/- 2 matching 105 male smokers and non-smokers. There was no significance on the comparison between female smokers and non-smokers. Smokers have significantly decreased serum alpha-tocopherol (1012 +/- 455, 1152 +/- 857, p < 0.03). The amount of serum ascorbate did not change. Smokers lowered serum HDL-cholesterol compared to non-smokers (59.3 +/- 11.8, 63.9 +/- 13.3, p < 0.05). The result of oxidative stress profile (OSP) also indicated that the increase of oxidative stress to smokers (p < 0.05). The calculated value of oxygen radical absorbance capacity (ORAC) of the meal for subjects was 1600 ORAC units.

摘要

针对吸烟者氧化应激的影响已经进行了大量研究。然而，似乎缺乏相关证据。通过分析1076名参与者（年龄35.9±12.9岁，尿8-羟基脱氧鸟苷均值±标准差，11.4±6.7，n = 1076），我们的研究发现吸烟者中DNA损伤生物标志物尿8-羟基脱氧鸟苷/肌酐显著增加（7.75±2.8 ng/ml×CRE（n = 154）和7.36±2.5 ng/ml×CRE（n = 627）（p < 0.05），相对风险=2.9（1.4 - 6.2），按性别和年龄±2匹配105名男性吸烟者和非吸烟者。女性吸烟者与非吸烟者之间的比较无显著差异。吸烟者血清α-生育酚显著降低（1012±455，1152±857，p < 0.03）。血清抗坏血酸含量未改变。与非吸烟者相比，吸烟者血清高密度脂蛋白胆固醇降低（59.3±11.8，63.9±13.3，p < 0.05）。氧化应激谱（OSP）结果也表明吸烟者氧化应激增加（p < 0.05）。受试者膳食的氧自由基吸收能力（ORAC）计算值为1600 ORAC单位。