Amoli Mahsa M, Garcia-Porrua Carlos, Ollier William E R, Gonzalez-Gay Miguel A
Centre for Integrated Genomic Medical Research, School of Epidemiology and Health Sciences, the University of Manchester, Manchester, UK.
J Rheumatol. 2005 Jan;32(1):74-6.
To investigate the role of macrophage migration inhibitory factor (MIF) gene polymorphism in giant cell arteritis (GCA).
Eighty-three patients with biopsy-proven GCA, 20 of them with visual ischemic complications, and 122 healthy matched controls from the Lugo region of Northwest Spain were studied. Patients and controls were genotyped for a single nucleotide polymorphism in the 5'-flanking region at position -173 of the MIF gene, using SNapshot ddNTP primer extension, followed by capillary electrophoresis (ABI 3100).
No significant differences in MIF gene polymorphism were observed in patients with biopsy-proven GCA compared to controls. This was also the case when GCA patients with or without visual ischemic complications were compared.
Polymorphism in MIF gene promoter -173 G/C does not appear to be a genetic risk factor for GCA in Northwest Spain.
探讨巨噬细胞移动抑制因子（MIF）基因多态性在巨细胞动脉炎（GCA）中的作用。
对西班牙西北部卢戈地区83例经活检证实的GCA患者（其中20例有视力缺血并发症）和122例健康对照者进行研究。采用SNapshot ddNTP引物延伸法，随后进行毛细管电泳（ABI 3100），对患者和对照者的MIF基因5'侧翼区-173位的单核苷酸多态性进行基因分型。
经活检证实的GCA患者与对照者相比，MIF基因多态性无显著差异。有或无视力缺血并发症的GCA患者之间比较也是如此。
在西班牙西北部，MIF基因启动子-173 G/C多态性似乎不是GCA的遗传危险因素。
