Liao Rongfeng, Gonzalez-Fernandez Federico
Department of Ophthalmology, University of Virginia, Charlottesville, USA.
Mol Vis. 2004 Dec 30;10:1038-46.
The interphotoreceptor matrix mediates interactions between the retinal pigment epithelium, photoreceptors, and Muller cells. Each of these cells contributes to specific proteoglycans, proteins, and growth factors in the interphotoreceptor matrix. Some components, such as interphotoreceptor retinoid binding protein (IRBP), are virtually unique to the interphotoreceptor matrix. It has been proposed that serum albumin, thought to be present in the interphotoreceptor matrix, could act as a surrogate retinoid binding protein within the subretinal space of transgenic mice lacking interphotoreceptor retinoid binding protein. To address this question, we sought to determine whether albumin is present in the interphotoreceptor matrix of IRBP+/+ mice or IRBP-/- mice.
We examined the distribution of albumin in IRBP-/- mice and IRBP+/+ mice using immunofluorescence and immunoperoxidase histochemistry.
The distribution of albumin within the corneal stroma, sclera, and capillaries is consistent with previous work. Serum albumin could not be detected in the interphotoreceptor matrix. The distribution of albumin in IRBP-/- mice was similar to that of their wildtype counterparts.
Serum albumin is not a component of the interphotoreceptor matrix of IRBP+/+ mice or IRBP-/- mice.
视网膜色素上皮、光感受器和穆勒细胞之间的相互作用由光感受器间基质介导。这些细胞各自对光感受器间基质中的特定蛋白聚糖、蛋白质和生长因子有贡献。某些成分，如光感受器间类视黄醇结合蛋白（IRBP），几乎是光感受器间基质所特有的。有人提出，被认为存在于光感受器间基质中的血清白蛋白，在缺乏光感受器间类视黄醇结合蛋白的转基因小鼠的视网膜下间隙中可作为替代类视黄醇结合蛋白发挥作用。为解决这个问题，我们试图确定白蛋白是否存在于IRBP+/+小鼠或IRBP-/-小鼠的光感受器间基质中。
我们使用免疫荧光和免疫过氧化物酶组织化学检查了IRBP-/-小鼠和IRBP+/+小鼠中白蛋白的分布。
白蛋白在角膜基质、巩膜和毛细血管中的分布与先前的研究一致。在光感受器间基质中未检测到血清白蛋白。IRBP-/-小鼠中白蛋白的分布与其野生型对应物相似。
血清白蛋白不是IRBP+/+小鼠或IRBP-/-小鼠光感受器间基质的组成成分。
