Wang Gang, Chen Ting-ting, Gao Chang-qing
Department of Cardiovascular Surgery, General Hospital of PLA, PLA Institute of Cardiac Surgery, Beijing 100853, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005 Jan;17(1):36-8.
To investigate the effects and mechanisms of ulinastatin on activities of myeloperoxidase (MPO) in lung tissue and neutrophil elastase (NE) in bronchoalveolar lavage fluid and to evaluate the protective effects of ulinastatin on rabbit lung in traumatic hemorrhagic shock.
Thirty rabbits were randomly assigned to three groups: control group, traumatic hemorrhagic shock group and ulinastatin-treatment group. The traumatic hemorrhagic shock model was reproduced by producing: femur fracture and femoral artery bleeding to reduce the mean artery pressure to (40+/-5) mmHg (1 mmHg=0.133 kPa). The hypotension was maintained 90 minutes before the shed blood and equivalent amount of Ringer's lactate was infused. Four hours after blood volume compensation, the activities of MPO in lung tissue and NE in bronchoalveolar lavage fluid (BALF) were measured, and the extravascular lung water volume was determined.
Compared with control group, the activities of either MPO in lung tissue or NE in BALF appeared to be increased in the ulinastatin-treatment group (both P<0.05), but their levels were significantly higher in traumatic hemorrhagic shock group(both P<0.05). The extravascular lung water volume was increased significantly in the two experimental group (both P<0.05), however it was more pronounced in traumatic hemorrhagic shock group (all P<0.05).
Ulinastatin can inhibit the increase in the activities of MPO in lung tissue and NE in BALF, and possesses potential protective effects on the lung tissue in traumatic hemorrhagic shock.
探讨乌司他丁对兔创伤失血性休克肺组织髓过氧化物酶（MPO）活性及支气管肺泡灌洗液中性粒细胞弹性蛋白酶（NE）活性的影响及其机制，评价乌司他丁对兔创伤失血性休克肺的保护作用。
将30只兔随机分为3组：对照组、创伤失血性休克组和乌司他丁治疗组。采用股骨骨折并股动脉放血致平均动脉压降至（40±5）mmHg（1 mmHg = 0.133 kPa）的方法复制创伤失血性休克模型。低血压状态维持90分钟后回输放出的血液并输注等量乳酸林格液。血容量补充4小时后，测定肺组织MPO活性及支气管肺泡灌洗液（BALF）中NE活性，并测定血管外肺水含量。
与对照组比较，乌司他丁治疗组肺组织MPO活性及BALF中NE活性均升高（均P < 0.05），但创伤失血性休克组上述指标升高更明显（均P < 0.05）。两实验组血管外肺水含量均显著增加（均P < 0.05），创伤失血性休克组更显著（均P < 0.05）。
乌司他丁可抑制创伤失血性休克时肺组织MPO活性及BALF中NE活性的升高，对创伤失血性休克时肺组织具有潜在的保护作用。
