[异丙酚降低大鼠肠缺血/再灌注后肺损伤中细胞间黏附分子-1的表达]

[Propofol reduces intercellular adhesion molecular-1 expression in lung injury following intestinal ischemia/reperfusion in rats].

作者信息

Hu Xiao-min, Lu Yang, Yao Shang-long

机构信息

Department of Anesthesiology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.

出版信息

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005 Jan;17(1):53-6.

PMID:15636717

Abstract

OBJECTIVE

To investigate the effect of propofol on intercellular adhesion molecular-1 (ICAM-1) expression in the lung tissue following intestinal ischemia/reperfusion (I/R) in rats.

METHODS

SD rats were randomly divided into 4 groups (n=8 in each group). (1) Group I/R in which rats were subjected to 1 hour of occlusion of the superior mesenteric artery (SMA), followed by 2 hours of reperfusion. (2) Early treatment group (group P1), rats were subjected to the same procedure as group I/R with the additional administration of propofol beginning 10 minutes before ischemia with 10 mg/kg loading dose, followed by continuous infusion at 10 mg(-1).kg(-1).h(-1). (3) Treatment group (group P2), rats were subjected identical insult as in group I/R with the administration of propofol started 10 minutes before reperfusion with 10 mg/kg loading dose, followed by continuous infusion at 10 mg.kg(-1).h(-1). (4) Sham-operation group, rats were subjected to laparotomy only, but received normal saline at 10 ml.kg(-1).h(-1). At the end of reperfusion, all animals were sacrificed. The activity of myeloperoxidase (MPO) and the content of tumor necrosis factor-alpha (TNF-alpha) were determined in the lung tissue, and plasma TNF-alpha content was also quantified. The ICAM-1 expression in pulmonary endothelium was assessed by histochemical staining.

RESULTS

All animals subjected to intestinal I/R demonstrated an increase in TNF-alpha in plasma and lung tissue, MPO activity and ICAM-1 expression in lung tissue. It was much more pronounced in I/R group. Plasma TNF-alpha content was increased significantly in group I/R and P2. All the increase was less in quantity in the early treatment group of propofol than the other two groups, and there was significant difference in contents of plasma TNF-alpha and ICAM-1 expression in lung between group I/R and group P1(both P<0.05).

CONCLUSION

ICAM-1 plays an important role in lung injury after intestinal I/R. The early treatment of propofol before intestinal I/R may be beneficial by reducing ICAM-1 expression in lung injury.

摘要

目的

探讨异丙酚对大鼠肠缺血/再灌注（I/R）后肺组织细胞间黏附分子-1（ICAM-1）表达的影响。

方法

将SD大鼠随机分为4组（每组n = 8）。（1）I/R组，大鼠接受肠系膜上动脉（SMA）阻断1小时，随后再灌注2小时。（2）早期治疗组（P1组），大鼠接受与I/R组相同的操作，在缺血前10分钟额外给予异丙酚，负荷剂量为10 mg/kg，随后以10 mg·kg⁻¹·h⁻¹持续输注。（3）治疗组（P2组），大鼠接受与I/R组相同的损伤，在再灌注前10分钟给予异丙酚，负荷剂量为10 mg/kg，随后以10 mg·kg⁻¹·h⁻¹持续输注。（4）假手术组，大鼠仅接受剖腹手术，但以10 ml·kg⁻¹·h⁻¹给予生理盐水。再灌注结束时，处死所有动物。测定肺组织中髓过氧化物酶（MPO）活性和肿瘤坏死因子-α（TNF-α）含量，并对血浆TNF-α含量进行定量。通过组织化学染色评估肺内皮细胞中ICAM-1的表达。

结果

所有接受肠I/R的动物血浆和肺组织中TNF-α均升高，肺组织中MPO活性和ICAM-1表达增加。在I/R组中更为明显。I/R组和P2组血浆TNF-α含量显著升高。异丙酚早期治疗组的所有升高量均低于其他两组，I/R组与P1组之间血浆TNF-α含量和肺中ICAM-1表达存在显著差异（均P < 0.05）。