Lai Feng-Jie, Huang Shiue-Shin, Hsieh Ming-Chia, Hsin Shih-Chie, Wu Chin-Han, Hsin Ya-Chieh, Shin Shyi-Jang
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung , Taiwan.
J Histochem Cytochem. 2005 Jan;53(1):45-53. doi: 10.1177/002215540505300106.
Experiments were performed to investigate whether adrenal neuronal nitric oxide synthase (nNOS) mRNA and protein expression are responsive to alterations in body volume. Using an RT-PCR technique, the relative quantities of nNOS mRNA as well as the tyrosine hydroxylase and phenylethanolamine N-methyltransferase mRNA in the adrenals of water-deprived rats significantly increased from 12 hr to 4 days. In situ hybridization and immunohistochemical study showed that water deprivation activated nNOS mRNA and protein expression in the adrenal medulla. Four days after water deprivation, nNOS protein expression determined by Western blot significantly increased in the adrenal gland. Our results are the first to demonstrate that nNOS syntheses in the adrenal medulla are markedly increased in water-deprived rats. This study also indicates that the upregulation of nNOS synthesis of the adrenal medulla is associated with the activation of adrenal medullary function in the face of volume depletion.
进行实验以研究肾上腺神经元型一氧化氮合酶（nNOS）的mRNA和蛋白表达是否对身体容量的改变有反应。使用逆转录聚合酶链反应（RT-PCR）技术，缺水大鼠肾上腺中nNOS mRNA以及酪氨酸羟化酶和苯乙醇胺N-甲基转移酶mRNA的相对量在12小时至4天内显著增加。原位杂交和免疫组织化学研究表明，缺水激活了肾上腺髓质中nNOS的mRNA和蛋白表达。缺水4天后，通过蛋白质免疫印迹法测定的肾上腺中nNOS蛋白表达显著增加。我们的结果首次证明，缺水大鼠肾上腺髓质中nNOS的合成显著增加。本研究还表明，肾上腺髓质中nNOS合成的上调与容量耗竭时肾上腺髓质功能的激活有关。
