Tan Yan, Tang Qiang, Hu Benrong, Xiang Jizhou
Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2004 Dec;10(12):890-3.
To further investigate the action mechanisms of berberine (Ber) and to assess the effects of Ber on the mRNA expression of phosphodiesterase type 5 (PDE5) in rat corpus cavernosum.
After incubating with Ber for 1 or 3 h respectively, we examined the levels of PDE5 mRNA by reverse transcription polymerase chain reaction (RT-PCR).
There were PDE5A1 and PDE5A2 mRNA expressions in the rat corpus cavernosum with PDE5A2 as the dominant isoform. Ber could obviously inhibit the mRNA expression of the two isoforms in the rat penis and bring on a pronounced decrease in PDE5A2 (P < 0.01).
The present study indicates that the inhibitory effect of Ber on PDE5 mRNA expression, especially on PDE5A2, might account for its molecular mechanism for treating ED.
进一步研究黄连素（Ber）的作用机制，并评估Ber对大鼠阴茎海绵体中5型磷酸二酯酶（PDE5）mRNA表达的影响。
分别用Ber孵育1小时或3小时后，通过逆转录聚合酶链反应（RT-PCR）检测PDE5 mRNA的水平。
大鼠阴茎海绵体中有PDE5A1和PDE5A2 mRNA表达，其中PDE5A2为主要亚型。Ber可明显抑制大鼠阴茎中这两种亚型的mRNA表达，并使PDE5A2显著降低（P < 0.01）。
本研究表明，Ber对PDE5 mRNA表达的抑制作用，尤其是对PDE5A2的抑制作用，可能是其治疗勃起功能障碍（ED）的分子机制。
