水稻细胞质核糖体蛋白基因OsRPS7的分子克隆与序列分析

[Molecular cloning and sequence analyzing of cytoplasmic ribosomal protein gene OsRPS7 from rice (Oryza sativa)].

作者信息

Gu Zhi-Min, Wang Jian-Fei, Huang Ji, Zhang Hong-Sheng

机构信息

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Yi Chuan. 2004 Mar;26(2):181-5.

PMID:15639984

Abstract

Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete Open Reading Frame (ORF) of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%,72% and 72% identity with those from Secale cereale, Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.

摘要

以黑麦细胞质核糖体蛋白ScRPS7的cDNA为查询探针，从华大水稻基因组数据库中获得了一个高度同源的水稻基因组重叠群。基于该重叠群，通过信息学方法组装了水稻细胞质核糖体蛋白S7的全长cDNA序列。此外，根据该组装的cDNA设计了两条引物，通过RT-PCR克隆了水稻核糖体蛋白的全长cDNA，并命名为OsRPS7。该cDNA长度为919bp，包含一个576bp的完整开放阅读框（ORF），编码一个由192个氨基酸残基组成的蛋白质。OsRPS7推导的氨基酸序列与黑麦、拟南芥和甘蓝的相应序列分别具有88%、72%和72%的同一性。本文分析了OsRPS7的基因组结构并对其功能进行了预测。