Kim J K, Wu R
Agricultural Biotechnology Institute, Rural Development Administration, Suwon, Republic of Korea.
Plant Mol Biol. 1993 Oct;23(2):409-13. doi: 10.1007/BF00029016.
A rice (Oryza sativa L.) cDNA clone coding for the cytoplasmic ribosomal protein L5, which associates with 5 S rRNA for ribosome assembly, was cloned and its nucleotide sequence was determined. The primary structure of rice L5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. These include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three repeated amino acid sequences; the conservation of glycine residues. This protein was identified as the nuclear-encoded cytoplasmic ribosomal protein L5 of rice by sequence similarity to other eucaryotic ribosomal 5 S RNA-binding proteins of rat, chicken, Xenopus laevis, and Saccharomyces cerevisiae. Rice L5 shares 51 to 62% amino acid sequence identity with the homologues. A group of ribosomal proteins from archaebacteria including Methanococcus vanniellii L18 and Halobacterium cutirubrum L13, which are known to be associated with 5 S rRNA, also related to rice L5 and the other eucaryotic counterparts, suggesting an evolutionary relationship in these ribosomal 5 S RNA-binding proteins.
克隆了一个编码细胞质核糖体蛋白L5的水稻（Oryza sativa L.）cDNA克隆，该蛋白与5S rRNA结合参与核糖体组装，并测定了其核苷酸序列。从核苷酸序列推导的水稻L5的一级结构包含294个氨基酸，具有一些有趣的特征，其中一些在其他真核生物同源物中也保守。这些特征包括：四个碱性氨基酸簇，其中一个可能作为核仁定位信号；三个重复的氨基酸序列；甘氨酸残基的保守性。通过与大鼠、鸡、非洲爪蟾和酿酒酵母的其他真核生物核糖体5S RNA结合蛋白的序列相似性，该蛋白被鉴定为水稻核编码的细胞质核糖体蛋白L5。水稻L5与同源物的氨基酸序列同一性为51%至62%。一组来自古细菌的核糖体蛋白，包括万氏甲烷球菌L18和红嗜盐菌L13，已知它们与5S rRNA相关，也与水稻L5和其他真核生物对应物相关，表明这些核糖体5S RNA结合蛋白之间存在进化关系。
