Geisslinger G, Menzel-Soglowek S, Schuster O, Brune K
Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, Germany.
J Chromatogr. 1992 Jan 3;573(1):163-7. doi: 10.1016/0378-4347(92)80492-9.
An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of flurbiprofen in human plasma is described. The procedure involved extraction of flurbiprofen from acidified plasma into hexane-diethyl ether (8:2, v/v). Stereoselective separation was achieved with a prepacked alpha 1-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine racemic flurbiprofen is also described. The detection wavelength was set at 246 nm. The limit of quantification was found to be 50 ng/ml for both assays. The method was demonstrated to be sufficiently sensitive for stereoselective pharmacokinetic studies of flurbiprofen.
