Castaño A, Hernández C
Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia, Spain.
Arch Virol. 2005 May;150(5):949-65. doi: 10.1007/s00705-004-0464-y. Epub 2005 Jan 13.
The complete nucleotide sequence of Pelargonium line pattern virus (PLPV) has been determined. The PLPV genomic RNA comprises 3884 nt and contains six open reading frames (ORFs) potentially encoding proteins of 27 (p27), 13 (p13), 87 (p87), 7 (p7), 6 (p6), and 37 kDa (p37), respectively. The arrangement of these ORFs on the PLPV genome closely resembles that of members of the genus Carmovirus in the family Tombusviridae and, moreover, most of the putative PLPV gene products showed high identity with proteins of this viral group. However, several striking differences were noticed. Carmoviruses generate two subgenomic RNAs whereas PLPV produces a single one. In addition, only p7 showed similarity with movement proteins of carmoviruses whereas p6 (as p13) has no viral (or other) homologs. This protein might be expressed from a non-canonical start codon or, alternatively, through a -1 frameshift (FS) mechanism. Both, the production of one subgenomic RNA and the likely involvement of a -1 FS for expression of an internal ORF parallel the translation strategies reported for the unique species of the genus Panicovirus, belonging also to the family Tombusviridae. Overall, the results support the placement of PLPV in this family although its peculiar characteristics preclude its direct assignment to any of the current genera.
天竺葵线条花纹病毒（PLPV）的完整核苷酸序列已被测定。PLPV基因组RNA由3884个核苷酸组成，包含六个开放阅读框（ORF），分别可能编码27 kDa（p27）、13 kDa（p13）、87 kDa（p87）、7 kDa（p7）、6 kDa（p6）和37 kDa（p37）的蛋白质。这些ORF在PLPV基因组上的排列与番茄病毒科番茄病毒属成员的排列非常相似，此外，大多数推测的PLPV基因产物与该病毒组的蛋白质具有高度同源性。然而，也注意到了一些显著差异。番茄病毒产生两个亚基因组RNA，而PLPV只产生一个。此外，只有p7与番茄病毒的运动蛋白相似，而p6（与p13一样）没有病毒（或其他）同源物。该蛋白质可能从非规范起始密码子表达，或者通过-1移码（FS）机制表达。产生一个亚基因组RNA以及可能通过-1 FS机制表达内部ORF，这两者都与番茄病毒科独特属的番木瓜病毒的翻译策略相似。总体而言，这些结果支持将PLPV归入该科，尽管其独特特征使其无法直接归入任何现有属。
