Liu W-K, Chu Y-L, Zhang F, Chen P, Cheng F, Wang H, Jia Y-Y, Ma T-Y
Department of Microbiology, Xi'an JiaoTong University, Xi'an Shaanxi Province, PR China.
Arch Virol. 2005 May;150(5):991-1001. doi: 10.1007/s00705-004-0454-0. Epub 2005 Jan 13.
The esophageal squamous cell carcinoma (ESCC) has high incidence in Shaanxi Province of China. More and more researches indicated that human papillomavirus type 16 (HPV16) might play an important role in carcinogenesis of ESCC but the relationship between HPV16 and CD44v6, nm23H1 has not been elucidated. HPV16 was detected by amplifying HPV16 E6 gene through polymerase chain reaction (PCR) method and the expression of CD44v6, nm23H1 in 40 ESCCs and fifteen normal esophageal mucosa (NEM) from Shaanxi Province was examined by Streptavidin-Peroxidase (SP) method using monoclonal antibody specific to CD44v6 and nm23H1. The positive rates of HPV16 E6 gene, CD44v6 and nm23H1 were 60% (24/40), 65% (26/40) and 45% (18/40) respectively in ESCCs and 26.67% (4/15), 33.33% (5/15) and 86.67% (13/15) respectively in NEMs. There exited statistical difference for HPV16, CD44v6 and nm23H1 between NEMs and ESCCs respectively (p < 0.05). The relationship between HPV16 and the expression of CD44v6 in ESCCs was statistical significance (P = 0.021), but no significant correlation was found between HPV16 and the expression of nm23H1 (P = 0.436) in ESCCs. The infection rate of HPV16 had no statistical difference in all pathological features we observed, but the expression rates of CD44v6 and nm23H1 had statistical correlation with invasion (p = 0.001, 0.013) and lymph nodes metastasis (p = 0.014, 0.002) respectively. In different histology grade of ESSCs, the relationship between HPV16 and CD44v6 was statistical significance in grade I (p = 0.044) but was not in grade II (p = 0.165) and grade III (p = 0.658), however as to the expression of nm23H1 there exited no statistical significance in all histology grades of ESCC (p > 0.05). The expression rates of CD44v6 and nm23H1 were statistically different between grade I and II (p = 0.004, 0.016) respectively and between grade I and grade III (p = 0.014, 0.020), but not statistically different between grade II and III (p = 0.792, 0.943) respectively. Our data firstly suggested that there existed the statistical relationship between the infection of HPV16 and the expression of CD44v6 in ESCCs and that HPV16 may be involved in invasion and metastasis of ESCC.
食管鳞状细胞癌（ESCC）在中国陕西省的发病率较高。越来越多的研究表明，16型人乳头瘤病毒（HPV16）可能在ESCC的致癌过程中发挥重要作用，但HPV16与CD44v6、nm23H1之间的关系尚未阐明。采用聚合酶链反应（PCR）法扩增HPV16 E6基因来检测HPV16，并使用针对CD44v6和nm23H1的单克隆抗体，通过链霉亲和素-过氧化物酶（SP）法检测40例来自陕西省的ESCC组织以及15例正常食管黏膜（NEM）中CD44v6、nm23H1的表达。ESCC中HPV16 E6基因、CD44v6和nm23H1的阳性率分别为60%（24/40）、65%（26/40）和45%（18/40），NEM中分别为26.67%（4/15）、33.33%（5/15）和86.67%（13/15）。NEM与ESCC之间HPV16、CD44v6和nm23H1的表达分别存在统计学差异（p<0.05）。ESCC中HPV16与CD44v6的表达之间具有统计学意义（P = 0.021），但HPV16与nm23H1的表达之间未发现显著相关性（P = 0.436）。在我们观察的所有病理特征中，HPV16的感染率无统计学差异，但CD44v6和nm23H1的表达率分别与浸润（p = 0.001，0.013）和淋巴结转移（p = 0.014，0.002）具有统计学相关性。在不同组织学分级的ESCC中，HPV16与CD44v6之间的关系在I级具有统计学意义（p = 0.044），而在II级（p = 0.165）和III级（p = 0.658）中无统计学意义，然而对于nm23H1的表达，在ESCC的所有组织学分级中均无统计学意义（p>0.05）。CD44v6和nm23H1的表达率在I级和II级之间（p = 0.004，0.016）以及I级和III级之间（p = 0.014，0.020）分别具有统计学差异，但在II级和III级之间（p = 0.792，0.943）分别无统计学差异。我们的数据首次表明，ESCC中HPV16感染与CD44v6表达之间存在统计学关系，并且HPV16可能参与ESCC的浸润和转移。
