Lévêque-Fort S, Fontaine-Aupart M P, Roger G, Georges P
Laboratoire de Photophysique Moléculaire, Centre National de la Recherche Scientifique, Unité Propre de Recherche 3361, Centre Universitaire, Batiment 210, 91405 Orsay, France.
Opt Lett. 2004 Dec 15;29(24):2884-6. doi: 10.1364/ol.29.002884.
Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for fluorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of fluorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample.
双光子显微镜是用于细胞或组织成像的强大工具。然而，它存在一个缺点，即作为一种激光扫描技术，荧光寿命成像的采集时间很长。因此，它通常仅限于强度图像，强度图像仅能给出荧光团的位置信息，但无法识别细胞成分之间的物理化学性质和相互作用。为了保护生物样品免受过长实验的影响，并提供一种更全面的光谱工具，我们开发了一种时间分辨多焦点多光子显微镜。这种设置使我们能够加快采集速度，同时保留测量样品强度图像和寿命图像的可能性。
