Beliakova T N, Glagolev A N, Skulachev V P
Biokhimiia. 1976 Aug;41(8):1478-83.
An uncoupler of oxidative phosphorylation causes an instantaneous cessation of movement of bacteria Rhodospirillum rubrum in the presence and in the absence of oligomycin. It is concluded that such cessation is not due to a decrease in the ATP concentration but to the elimination of deltamicron-H+ by the uncoupler. The mobility of the bacteria does not practically change in the presence of acetate and is, to some extent, decreased after addition of valinomycin or penetrating cation of tetraphenyl phosphonium. Under a combined action of acetate and valinomycin the movement is depleted. It is concluded that both constituents of deltamicronH+-transmembrane difference of electric potentials and the pH gradient--may serve as energy sources for the bacteria movement. Inhibitory analysis data suggest that the bacteria movement may be maintained by any of the deltamicronH+ sources, e.g. light-dependent cyclic electron transfer, respiration, ATPase and membrane pyrophosphatase.
氧化磷酸化解偶联剂会使深红螺菌在有和没有寡霉素的情况下瞬间停止运动。得出的结论是，这种停止并非由于ATP浓度降低，而是由于解偶联剂消除了ΔμH⁺。在存在乙酸盐的情况下，细菌的运动性实际上没有变化，而在添加缬氨霉素或四苯基鏻穿透性阳离子后，运动性在一定程度上有所降低。在乙酸盐和缬氨霉素的共同作用下，运动会耗尽。得出的结论是，ΔμH⁺跨膜电势差的两个组成部分以及pH梯度都可以作为细菌运动的能量来源。抑制分析数据表明，细菌的运动可以由任何ΔμH⁺来源维持，例如光依赖的循环电子传递、呼吸作用、ATP酶和膜焦磷酸酶。
