Harayama S, Iino T
J Bacteriol. 1977 Jul;131(1):34-41. doi: 10.1128/jb.131.1.34-41.1977.
Cells of the photosynthetic bacterium Rhodospirillum rubrum cultivated anaerobically in light show phototaxis. The behavior of individual cells in response to the phenomenon is reversal(s) of the swimming direction when the intensity of the light available to them abruptly decreases. The tactic response was inhibited by antimycin, an inhibitor of the photosynthetic electron transfer system. The inhibitory effect of antimycin was overcome by phenazine methosulfate. Motility of the cells was not impaired by antimycin under aerobic conditions. Valinomycin plus potassium also inhibited their phototactic response; however, valinomycin or potassium alone had no effect. A change in membrane potential of the cells was measured as an absorbance change of carotenoid. Changes in the membrane potential caused by "on-off" light were prevented by antimycin and by valinomycin plus potassium, but not by antimycin plus phenazine methosulfate nor valinomycin or potassium alone. The results indicated that the phototactic response of R. rubrum is mediated by a sudden change in electron flow in the photosynthetic electron transfer system, and that the membrane potential plays an important role in manifestation of the response.
在光照下厌氧培养的光合细菌红螺菌细胞表现出趋光性。单个细胞对这种现象的反应是，当它们可获得的光照强度突然降低时，游泳方向发生反转。趋化反应受到抗霉素（一种光合电子传递系统抑制剂）的抑制。吩嗪硫酸甲酯可克服抗霉素的抑制作用。在有氧条件下，抗霉素不会损害细胞的运动能力。缬氨霉素加钾也会抑制它们的趋光反应；然而，单独的缬氨霉素或钾没有作用。细胞的膜电位变化通过类胡萝卜素的吸光度变化来测量。由“开-关”光引起的膜电位变化被抗霉素和缬氨霉素加钾阻止，但不被抗霉素加吩嗪硫酸甲酯或单独的缬氨霉素或钾阻止。结果表明，红螺菌的趋光反应是由光合电子传递系统中电子流的突然变化介导的，并且膜电位在该反应的表现中起重要作用。
