脂联素及其他脂肪细胞因子作为富含甘油三酯脂蛋白代谢标志物的预测指标。

Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.

作者信息

Chan Dick C, Watts Gerald F, Ng Theodore W K, Uchida Yoshiaki, Sakai Naohiko, Yamashita Shizuya, Barrett P Hugh R

机构信息

School of Medicine and Pharmacology, Western Australian Institute for Medical Research, University of Western Australia, Perth, Western Australia, Australia.

出版信息

Clin Chem. 2005 Mar;51(3):578-85. doi: 10.1373/clinchem.2004.045120. Epub 2005 Jan 13.

DOI:10.1373/clinchem.2004.045120

PMID:15650029

Abstract

BACKGROUND

Adipocytokines are bioactive peptides that may play an important role in the regulation of glucose and lipid metabolism. In this study, we investigated the association of plasma adipocytokine concentrations with markers of triglyceride-rich lipoprotein (TRL) metabolism in men.

METHODS

Fasting adiponectin, leptin, resistin, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), apolipoprotein (apo) B-48, apo C-III, and remnant-like particle (RLP)-cholesterol concentrations were measured by immunoassays and insulin resistance by homeostasis assessment (HOMA) score in 41 nondiabetic men with a body mass index of 22-35 kg/m2. Visceral and subcutaneous adipose tissue masses (ATMs) were determined by magnetic resonance imaging and total ATM by bioelectrical impedance.

RESULTS

In univariate regression, plasma adiponectin and leptin concentrations were inversely and directly associated with plasma apoB-48, apoC-III, RLP-cholesterol, triglycerides, VLDL-apoB, and VLDL-triglycerides (P <0.05). Resistin, IL-6, and TNF-alpha were not significantly associated with any of these variables, except for a direct correction between apoC-III and IL-6 (P <0.05). In multivariate regression including HOMA, age, nonesterified fatty acids, and adipose tissue compartment, adiponectin was an independent predictor of plasma apoB-48 (beta coefficient = -0.354; P = 0.048), apoC-III (beta coefficient = -0.406; P = 0.012), RLP-cholesterol (beta coefficient = -0.377; P = 0.016), and triglycerides (beta coefficient = -0.374; P = 0.013). By contrast, leptin was not an independent predictor of these TRL markers. Plasma apoB-48, apoC-III, RLP-cholesterol, and triglycerides were all significantly and positively associated with plasma insulin, HOMA, and visceral, subcutaneous, and total ATMs (P <0.05).

CONCLUSIONS

These data suggest that the plasma adiponectin concentration may not only link abdominal fat, insulin resistance, and dyslipidemia, but may also exert an independent role in regulating TRL metabolism.

摘要

背景

脂肪细胞因子是生物活性肽，可能在葡萄糖和脂质代谢调节中发挥重要作用。在本研究中，我们调查了男性血浆脂肪细胞因子浓度与富含甘油三酯脂蛋白（TRL）代谢标志物之间的关联。

方法

采用免疫分析法测定了41名体重指数为22 - 35 kg/m²的非糖尿病男性的空腹脂联素、瘦素、抵抗素、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、载脂蛋白（apo）B-48、apo C-III和残粒样颗粒（RLP）-胆固醇浓度，并通过稳态评估（HOMA）评分评估胰岛素抵抗。通过磁共振成像测定内脏和皮下脂肪组织质量（ATM），通过生物电阻抗测定总ATM。

结果

在单变量回归中，血浆脂联素和瘦素浓度分别与血浆apoB-48、apoC-III、RLP-胆固醇、甘油三酯、极低密度脂蛋白（VLDL）-apoB和VLDL-甘油三酯呈负相关和正相关（P <0.05）。抵抗素、IL-6和TNF-α与这些变量均无显著相关性，但apoC-III与IL-6之间存在直接相关性（P <0.05）。在包括HOMA、年龄、非酯化脂肪酸和脂肪组织分区的多变量回归中，脂联素是血浆apoB-48（β系数 = -0.354；P = 0.048）、apoC-III（β系数 = -0.406；P = 0.012）、RLP-胆固醇（β系数 = -0.377；P = 0.016）和甘油三酯（β系数 = -0.374；P = 0.013）的独立预测因子。相比之下，瘦素不是这些TRL标志物的独立预测因子。血浆apoB-48、apoC-III、RLP-胆固醇和甘油三酯均与血浆胰岛素、HOMA以及内脏、皮下和总ATM显著正相关（P <0.05）。