西班牙首次成功建立人类胚胎干细胞系：使用长期冷冻保存的胚胎及无动物条件。
First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions.
作者信息
Simón Carlos, Escobedo Carmen, Valbuena Diana, Genbacev Olga, Galan Amparo, Krtolica Ana, Asensi Angeles, Sánchez Eva, Esplugues Juan, Fisher Susan, Pellicer Antonio
机构信息
Valencia Stem Cell Bank, Centro Superior de Alta Tecnología, Valencia, Spain.
出版信息
Fertil Steril. 2005 Jan;83(1):246-9. doi: 10.1016/j.fertnstert.2004.09.004.
The first two human embryonic stem cell lines (VAL-1 and VAL-2) have been derived in Spain with long-term cryopreserved embryos under animal-free conditions. In the first series, 40 human embryos that had been cryopreserved at day 2 of development were thawed after >5 years. A derivation efficiency of 5% per frozen embryo or 12.5% per blastocyst was obtained.
西班牙已在无动物条件下，利用长期冷冻保存的胚胎培育出了首批两个人类胚胎干细胞系（VAL-1和VAL-2）。在首个系列实验中，40个发育至第2天便被冷冻保存的人类胚胎在5年多后被解冻。每个冷冻胚胎的衍生效率为5%，每个囊胚的衍生效率为12.5%。
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