SNL 成纤维细胞饲养层支持人诱导多能干细胞的衍生和维持。
SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.
机构信息
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.
出版信息
J Genet Genomics. 2010 Apr;37(4):241-8. doi: 10.1016/S1673-8527(09)60042-4.
Abstract
Induced pluripotent stem (iPS) cells can be derived from human somatic cells by cellular reprogramming. This technology provides a potential source of non-controversial therapeutic cells for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts (MEFs) are used as feeder layers in the initial derivation of iPS lines. The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, displayed human embryonic stem cells (hESCs) morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.
摘要
诱导多能干细胞（iPS）可通过细胞重编程从人体细胞中获得。该技术为组织修复、药物发现以及研究人类疾病分子基础提供了一种非争议性治疗细胞的潜在来源。通常，鼠胚胎成纤维细胞（MEFs）被用作 iPS 系最初衍生的饲养层。本研究旨在确定 SNL 成纤维细胞是否可用于支持使用慢病毒表达的重编程因子从体细胞重编程的人 iPS 细胞的生长。在我们的研究中，iPS 细胞表达常见的多能性标记物，显示出人类胚胎干细胞（hESCs）的形态和 NANOG 和 OCT4 的未甲基化启动子。这些数据表明 SNL 饲养细胞可支持人 iPS 细胞的衍生和维持。
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