烟草中Q染色体特异性DNA标记的开发及其在烟草单体系鉴定中的应用。
Development of Q-chromosome-specific DNA markers in tobacco and their use for identification of a tobacco monosomic line.
作者信息
Tezuka Takahiro, Onosato Katsura, Hijishita Saori, Marubashi Wataru
机构信息
School of Agriculture, Ibaraki University, Ami, Ibaraki, 300-0393 Japan.
出版信息
Plant Cell Physiol. 2004 Dec;45(12):1863-9. doi: 10.1093/pcp/pch204.
We developed seven Q-chromosome-specific DNA markers in Nicotiana tabacum by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis using two hybrid lines, and we were able to identify tobacco monosomic plants among F1 progeny derived from the cross N. tabacum Haplo-QxN. tabacum cv. Samsun NN using Q-chromosome-specific DNA markers. Based on the results, we discuss the roles of the Q chromosome in embryo sac development and embryogenesis. Here, we propose a new method for identifying DNA markers for a particular chromosome in the genus Nicotiana.
我们通过随机扩增多态性DNA（RAPD）和简单序列重复区间（ISSR）分析，利用两个杂交系在烟草中开发了7个Q染色体特异性DNA标记，并且能够使用Q染色体特异性DNA标记，在烟草单倍体-Q×烟草品种萨姆松NN杂交产生的F1后代中鉴定出烟草单体植株。基于这些结果，我们讨论了Q染色体在胚囊发育和胚胎发生中的作用。在此，我们提出了一种鉴定烟草属特定染色体DNA标记的新方法。
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