来自蠓科咬蠓的无鬃螺旋体新种

Spiroplasma atrichopogonis sp. nov., from a ceratopogonid biting midge.

作者信息

Koerber R Thomas, Gasparich Gail E, Frana Mark F, Grogan William L

机构信息

Department of Biological Sciences, Towson University, Towson, MD 21252, USA.

Department of Biological Sciences, Salisbury University, Salisbury, MD 21801, USA.

出版信息

Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):289-292. doi: 10.1099/ijs.0.02465-0.

DOI:10.1099/ijs.0.02465-0

PMID:15653889

Abstract

Spiroplasma sp. strain GNAT3597T was isolated from the biting midge genus Atrichopogon (Diptera: Ceratopogonidae). It was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a cell membrane (i.e. lacking a cell wall). Growth of strain GNAT3597T occurred in M1D medium at 30 degrees C. Strain GNAT3597T catabolized both glucose and arginine, but did not hydrolyse urea. The DNA G+C content of strain GNAT3597T was 29+/-1 mol%. Only one strain, SMCAT (Spiroplasma mirum), is serologically related to strain GNAT3597T, although the relationship is weak (positive reaction to only a 1 : 80 dilution). It is therefore proposed that strain GNAT3597T (=ATCC BAA-520T=NBRC 100390T) represents a novel species, Spiroplasma atrichopogonis sp. nov. (class Mollicutes: order Entomoplasmatales: family Spiroplasmataceae).

摘要

螺原体属菌株GNAT3597T是从蠛蠓属（双翅目：蠓科）的吸血蠓中分离得到的。它在血清学上与其他螺原体物种、类群或亚群不同。细胞的暗视野显微镜观察显示出典型的螺旋形状，随后的透射电子显微镜观察显示细胞仅被细胞膜包围（即没有细胞壁）。菌株GNAT3597T在30℃的M1D培养基中生长。菌株GNAT3597T能分解葡萄糖和精氨酸，但不水解尿素。菌株GNAT3597T的DNA G+C含量为29±1 mol%。只有一个菌株SMCAT（微小螺原体）与菌株GNAT3597T有血清学关系，尽管这种关系很弱（仅对1:80稀释度有阳性反应）。因此，建议将菌株GNAT3597T（=ATCC BAA-520T=NBRC 100390T）代表一个新物种，即蠛蠓螺原体（Spiroplasma atrichopogonis sp. nov.）（柔膜菌纲：昆虫原体目：螺原体科）。