新种速生螺原体，源自胡蜂四齿独栖蜂。

Spiroplasma velocicrescens sp. nov., from the vespid wasp Monobia quadridens.

作者信息

Konai M, Whitcomb R F, Tully J G, Rose D L, Carle P, Bové J M, Henegar R B, Hackett K J, Clark T B, Williamson D L

机构信息

Insect Biocontrol Laboratory, Beltsville Agricultural Research Center, U.S. Department of Agriculture, Beltsville, Maryland 20705.

出版信息

Int J Syst Bacteriol. 1995 Apr;45(2):203-6. doi: 10.1099/00207713-45-2-203.

DOI:10.1099/00207713-45-2-203

PMID:7727271

Abstract

Spiroplasma strain MQ-4T (T = type strain), which was isolated from the hemolymph of the vespid wasp Monobia quadridens, was serologically distinct from other spiroplasma species, groups, putative groups, and subgroups. Each strain MQ-4T cell was helical and motile and was surrounded by a single cytoplasmic membrane; there was no evidence of a cell wall. The strain grew well in 1% serum fraction medium, as well as in SM-1, M1D, and SP-4 liquid media, under both aerobic and anaerobic conditions. Strain MQ-4T grew at temperatures ranging from 10 to 41 degrees C but did not grow at 43 degrees C. The strain grew optimally at 37 degrees C with a doubling time of 0.6 h, the shortest doubling time recorded for any spiroplasma. Strain MQ-4T catabolized glucose and arginine but did not hydrolyze urea. The guanine-plus-cytosine content of the DNA was about 27.5 +/- 1 mol%. The genome size was 1,480 kbp (940 MDa). Strain MQ-4 (= ATCC 35262) is designated the type strain of a new species, Spiroplasma velocicrescens.

摘要

从胡蜂四齿独栖蜂（Monobia quadridens）血淋巴中分离出的螺旋体菌株MQ - 4T（T = 模式菌株），在血清学上与其他螺旋体物种、类群、假定类群和亚群不同。每个MQ - 4T菌株细胞呈螺旋状且具运动性，被单一细胞质膜包围；没有细胞壁存在的证据。该菌株在1%血清成分培养基以及SM - 1、M1D和SP - 4液体培养基中，在需氧和厌氧条件下均生长良好。菌株MQ - 4T在10至41摄氏度的温度范围内生长，但在43摄氏度时不生长。该菌株在37摄氏度时生长最佳，倍增时间为0.6小时，这是所记录的任何螺旋体最短的倍增时间。菌株MQ - 4T分解代谢葡萄糖和精氨酸，但不水解尿素。DNA的鸟嘌呤加胞嘧啶含量约为27.5 +/- 1摩尔%。基因组大小为1480 kbp（940 MDa）。菌株MQ - 4（= ATCC 35262）被指定为新物种快速生长螺旋体（Spiroplasma velocicrescens）的模式菌株。