Thawai Chitti, Tanasupawat Somboon, Itoh Takashi, Suwanborirux Khanit, Suzuki Ken-Ichiro, Kudo Takuji
Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):417-422. doi: 10.1099/ijs.0.63217-0.
Two actinomycete strains, LK2-10T and LK2-5, which produced single, non-motile spores, were isolated from peat swamp forest soil in Yala Province, Thailand. A polyphasic study was carried out to establish the taxonomic position of these strains. Morphological and chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora. Phylogenetic analysis using 16S rRNA gene sequences also indicated that these strains should be classified in the genus Micromonospora and clearly separated from their closest relative, Micromonospora nigra DSM 43818T. Furthermore, a combination of DNA-DNA hybridization results and physiological and biochemical properties indicated that these strains were distinguished from all recognized Micromonospora species. These strains therefore represent a novel species, for which the name Micromonospora eburnea sp. nov. is proposed. The type strain is LK2-10T (=JCM 12345T=PCU 238T=DSM 44814T=TISTR 1531T).
从泰国也拉府的泥炭沼泽森林土壤中分离出两株放线菌菌株LK2-10T和LK2-5，它们产生单个、不运动的孢子。开展了多相分类研究以确定这些菌株的分类地位。这些菌株的形态和化学分类特征与小单孢菌属的特征一致。使用16S rRNA基因序列进行的系统发育分析也表明，这些菌株应归入小单孢菌属，并且与它们关系最近的亲缘种黑小单孢菌DSM 43818T明显分开。此外，DNA-DNA杂交结果与生理生化特性相结合表明，这些菌株与所有已确认的小单孢菌物种都不同。因此，这些菌株代表一个新物种，为此提议将其命名为白小单孢菌（Micromonospora eburnea sp. nov.）。模式菌株为LK2-10T（=JCM 12345T=PCU 238T=DSM 44814T=TISTR 1531T）。
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