利用随机扩增多态性DNA（RAPD）技术对红色毛癣菌进行菌株鉴别
Strain differentiation of Trichophyton rubrum by random amplification of polymorphic DNA (RAPD).
作者信息
Baeza Lilian Cristiane, Giannini Maria José Soares Mendes
机构信息
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, 14801-902 Araraquara, SP, Brazil.
出版信息
Rev Inst Med Trop Sao Paulo. 2004 Nov-Dec;46(6):339-41. doi: 10.1590/s0036-46652004000600008. Epub 2005 Jan 10.
Trichophyton rubrum is an important cause of dermatomycoses. Molecular strain typing methods have recently been developed to address questions about epidemiology and source of relapse following treatment. This report describes the application of RAPD for molecular strain differentiation of this fungus utilizing the primers 1- (5'-d[GGTGCGGGAA]-3') and 6- (5'-d[CCCGTCAGCA]-3'). A total of five RAPD patterns were observed among 10 strains of T. rubrum, with each of the primers used. We conclude that RAPD analysis using primers 1 and 6 can be used in epidemiological studies.
红色毛癣菌是皮肤癣菌病的重要病因。最近已开发出分子菌株分型方法，以解决有关治疗后流行病学和复发来源的问题。本报告描述了利用引物1-（5'-d[GGTGCGGGAA]-3'）和6-（5'-d[CCCGTCAGCA]-3'）通过随机扩增多态性DNA（RAPD）对该真菌进行分子菌株分化的应用。在10株红色毛癣菌中，使用每种引物共观察到5种RAPD模式。我们得出结论，使用引物1和6的RAPD分析可用于流行病学研究。
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