Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, PO Box 486, Belo Horizonte, Minas Gerais, Brazil.
Mycopathologia. 2010 Apr;169(4):247-55. doi: 10.1007/s11046-009-9261-1. Epub 2009 Nov 25.
Onychomycosis is a common condition that represents up to 50% of all nail problems and 30% of all cases of dermatophytoses. Trichophyton rubrum and Trichophyton interdigitale are the most common agents involved in this condition. In cases of recurrent post-treatment onychomycosis, strain fingerprinting could reveal whether the original isolate is responsible, a new strain has been acquired or if multiple strains are involved. The aim of this study was to evaluate the efficacy of the RAPD method for species and strain differentiation of T. rubrum and T. interdigitale obtained from patients with subungeal distal-lateral onychomycosis. A set of 86 strains of onychomycosis causative dermatophytes were submitted to species differentiation and strain typing by RAPD method with two previously described primers. Both primers proved capable of strain differentiation when tested for each species. Nineteen molecular profiles were configured for T. rubrum isolates with primers 1 and 6. For T. mentagrophytes, ten molecular profiles were configured with primer 1 and twenty-one with primer 6. We found that T. interdigitale and T. rubrum species were grouped in different clusters when both primers were analyzed together. This study shows that these primers are valuable tools for strain differentiation with T. rubrum and T. intedigitale.
甲真菌病是一种常见疾病，占所有指甲问题的 50%，占所有皮肤癣菌病的 30%。红色毛癣菌和须癣毛癣菌是引起这种疾病的最常见的病原体。在治疗后复发性甲真菌病的情况下，菌株指纹分析可以揭示是否是原来的分离物负责，是否获得了新的菌株，或者是否涉及多种菌株。本研究旨在评估 RAPD 方法对趾甲远端侧下甲真菌病患者的红色毛癣菌和须癣毛癣菌进行种和菌株分化的疗效。用两种先前描述的引物对 86 株趾甲真菌病致病皮肤癣菌进行了 RAPD 法的种分化和菌株分型。当对每种真菌进行测试时，两种引物都证明能够进行菌株分化。用引物 1 和 6 为红色毛癣菌分离株配置了 19 种分子图谱。用引物 1 为须癣毛癣菌配置了 10 种分子图谱，用引物 6 配置了 21 种分子图谱。我们发现，当同时分析两种引物时，红色毛癣菌和须癣毛癣菌被分为不同的聚类。这项研究表明，这些引物是对红色毛癣菌和须癣毛癣菌进行菌株分化的有用工具。
