[双参通冠（SSTG）对心肌缺血再灌注损伤时肿瘤坏死因子-α、细胞间黏附分子-1的影响]
[Effects of shuangshen tongguan (SSTG) on TNF-alpha, ICAM-1 during myocardial ischemia-reperfusion injury].
作者信息
Han Xiao, Liu Jian-xun, Ma Xiao-bin, Wang Yang-hui
机构信息
Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Nov;29(11):1073-5.
OBJECTIVE
To observe the effects of SSTG on infarction size and tumor necrosis factor-alpha (TNF-alpha), intercellular adhesion molecular-1 (ICAM-1) levels in serum during reperfusion injury of acute myocardial ischemia.
METHOD
Anterior descending branch of coronary artery was ligated and released to create myocardial ischemia-reperfusion injury. The size and weight of infarction area and the contents of TNF-alpha, IGAM-1 in serum were assayed by N-BT staining and ELISA respectively.
RESULT
The size and weight of infarct area and the contents of TNF-alpha, ICAM-1 in serum were significantly increased compared with the normal group and were obviously decreased after being treated with SSIG.
CONCLUSION
Ischemia-reperfusion stimulated the secretion of TNF-alpha and ICAM-1, which play an important role in ischemia-reperfusion injury. SSTG might protect myocardium from ischemia-reperfusion injury by suppressing over-secretion of TNF-alpha and ICAM-1 and reducing the size and weight of infarction area.
目的
观察自体外周血干细胞移植（SSTG）对急性心肌缺血再灌注损伤时梗死面积及血清肿瘤坏死因子-α（TNF-α）、细胞间黏附分子-1（ICAM-1）水平的影响。
方法
结扎并松开冠状动脉前降支以制造心肌缺血再灌注损伤。分别采用N-BT染色法和酶联免疫吸附测定法（ELISA）检测梗死面积的大小和重量以及血清中TNF-α、ICAM-1的含量。
结果
与正常组相比，梗死面积的大小和重量以及血清中TNF-α、ICAM-1的含量显著增加，而经自体外周血干细胞移植（SSIG）治疗后明显降低。
结论
缺血再灌注刺激TNF-α和ICAM-1的分泌，它们在缺血再灌注损伤中起重要作用。自体外周血干细胞移植（SSTG）可能通过抑制TNF-α和ICAM-1的过度分泌以及减小梗死面积的大小和重量来保护心肌免受缺血再灌注损伤。
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