在直链淀粉三（3,5-二甲基苯基氨基甲酸酯）手性固定相上通过高效液相色谱法对阿苯达唑亚砜对映体进行分析和半制备拆分

Analytical and semipreparative resolution of enatiomers of albendazole sulfoxide by HPLC on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases.

作者信息

Zongde Zhai, Xingping Luo, Xiaomei Wu, Hong Zhang, Yanping Shi, Liren Chen, Yongmin Li

机构信息

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

出版信息

J Biochem Biophys Methods. 2005 Jan 31;62(1):69-79. doi: 10.1016/j.jbbm.2004.09.006. Epub 2004 Dec 19.

DOI:10.1016/j.jbbm.2004.09.006

PMID:15656945

Abstract

Broad spectrum anthelmintic agent-albendazole sulfoxide (ABZSO) have been separated and semiprepared on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases by HPLC using mobile phases contained with n-hexane and different alcohols. For analytical separation the influence of the nature and content of alcoholic modifiers on separation were systemically studied. Then, the analytical methods were scaled up to semipreparative loading to obtain small quantities (about 1 g) of both ABZSO enantiomers. Especially, different loading amounts were investigated for their effect on various parameters of semipreparative HPLC. In addition, optical rotation and circular dichroism (CD) of both ABZSO enantiomers collected were determined and single enantiomers were found stable in configuration for 1 year.

摘要

广谱驱虫剂阿苯达唑亚砜（ABZSO）已通过高效液相色谱法在直链淀粉三（3,5-二甲基苯基氨基甲酸酯）手性固定相上进行分离和半制备，使用含有正己烷和不同醇类的流动相。对于分析分离，系统研究了醇类改性剂的性质和含量对分离的影响。然后，将分析方法放大至半制备进样量，以获得少量（约1 g）的两种ABZSO对映体。特别地，研究了不同进样量对半制备高效液相色谱各种参数的影响。此外，对收集的两种ABZSO对映体的旋光性和圆二色性（CD）进行了测定，发现单一对映体在构型上稳定1年。