Zhai Zongde, Zhang Hong, Dun Huijuan, Liu Chunhui, Chen Liren, Li Yongmin
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China.
Se Pu. 2004 May;22(3):244-7.
A chiral stationary phase (CSP) was prepared by coating amylose-tris(3,5-dimethylphenyl carbamate) (ADMPC) onto aminopropylated spherical silica gel. Albendazole sulfoxide (ABZSO) was directly resolved on the ADMPC-CSP by normal-phase high performance liquid chromatography (HPLC) using a series of binary and ternary mobile phases. The results indicated that the retention and stereoselectivity of ABZSO on ADMPC-CSP were significantly influenced by the kinds and the contents of the alcohols in mobile phases. The use of methanol and/or ethanol as alcoholic modifier achieved better separation of ABZSO. Effective and efficient chiral resolution was obtained by using ternary mobile phases: hexane/2-propanol/methanol or hexane/ethanol/methanol.
通过将直链淀粉-三（3,5-二甲基苯基氨基甲酸酯）（ADMPC）涂覆在氨丙基化球形硅胶上制备了一种手性固定相（CSP）。使用一系列二元和三元流动相，通过正相高效液相色谱（HPLC）在ADMPC-CSP上直接拆分阿苯达唑亚砜（ABZSO）。结果表明，流动相中醇的种类和含量对ABZSO在ADMPC-CSP上的保留和立体选择性有显著影响。使用甲醇和/或乙醇作为醇类改性剂可实现ABZSO的更好分离。通过使用三元流动相：己烷/2-丙醇/甲醇或己烷/乙醇/甲醇，获得了有效且高效的手性拆分。
