[Influence of mobile phase composition on chiral separation of albendazole sulfoxide racemates].

A chiral stationary phase (CSP) was prepared by coating amylose-tris(3,5-dimethylphenyl carbamate) (ADMPC) onto aminopropylated spherical silica gel. Albendazole sulfoxide (ABZSO) was directly resolved on the ADMPC-CSP by normal-phase high performance liquid chromatography (HPLC) using a series of binary and ternary mobile phases. The results indicated that the retention and stereoselectivity of ABZSO on ADMPC-CSP were significantly influenced by the kinds and the contents of the alcohols in mobile phases. The use of methanol and/or ethanol as alcoholic modifier achieved better separation of ABZSO. Effective and efficient chiral resolution was obtained by using ternary mobile phases: hexane/2-propanol/methanol or hexane/ethanol/methanol.