Shilova O B, Markina N V, Perepelkina O V, Gichenok I V, Korochkin L I, Poletaeva I I
Zh Vyssh Nerv Deiat Im I P Pavlova. 2004 Nov-Dec;54(6):785-94.
DBA/2, CBA mice, and their F1 hybrids (first series) and 101/HY and C3H mice (second series) were injected as neonates (2-7 days of life) with Semax (sc., 7 microg per animal). Semax is a peptide analogue of ACHT4-10 fragment which is resistant to degradation. The common feature of remote effects of both Semax and saline injections was the set of changes in the open-field behavior in adult (2.5- to 3-month-old) animals as compared to intact mice. Unexpectedly, the neonatal saline injections induced many changes in adult behavior, part of these effects being genotype-dependent. The most conspicuous shifts (genotype-dependent increase or decline) in freezing, grooming and rearing scores were displayed by DBA/2 and C3H mice, whereas the hole-poke frequencies were significantly changed in CBA and C3H mice. Squares crossed in the center of arena and rearing number were significantly increased in saline group of DBA/2 mice, whereas in Semax-injected DBA/2 group they were approximately equal to the level of intact mice. This means that the remote effects of noxious stimulation (injections of saline) were in some ways "compensated" as the result of concomitant peptide effect. At the same time, the numbers of freezing and grooming episodes were also increased in these groups. Because exploratory behavior and manifestations of anxiety increased or decreased simultaneously, it proves to be difficult to ascribe these changes to behavioral modulation along the "novelty seeking--anxiety" axis. In mice of other genotypes, changes in the same indices of the open-field behavior were revealed, but these changes were different in their direction. It was suggested that the complex patterns of postnatal behavior was the result of neonatal injections modulating subsequent brain development.
将新生小鼠（出生2 - 7天）的DBA/2、CBA小鼠及其F1代杂种（第一组）以及101/HY和C3H小鼠（第二组）皮下注射Semax（每只动物7微克）。Semax是一种对降解具有抗性的ACHT4 - 10片段的肽类似物。与未处理的小鼠相比，Semax和生理盐水注射的远期效应的共同特征是成年（2.5至3个月大）动物在旷场行为中的一系列变化。出乎意料的是，新生期生理盐水注射诱导了成年行为的许多变化，其中部分效应是基因型依赖性的。DBA/2和C3H小鼠在僵住、梳理和直立评分方面表现出最明显的变化（基因型依赖性增加或减少），而CBA和C3H小鼠的洞戳频率有显著变化。DBA/2小鼠生理盐水组在竞技场中心穿过的方格数和直立次数显著增加，而在注射Semax的DBA/2组中，它们与未处理小鼠的水平大致相当。这意味着有害刺激（注射生理盐水）的远期效应在某种程度上因伴随的肽效应而“得到补偿”。同时，这些组中的僵住和梳理次数也增加了。由于探索行为和焦虑表现同时增加或减少，因此很难将这些变化归因于沿“新奇寻求 - 焦虑”轴的行为调节。在其他基因型的小鼠中，也发现了旷场行为相同指标的变化，但这些变化的方向不同。有人提出，出生后行为的复杂模式是新生期注射调节后续大脑发育的结果。
