文昌鱼FoxQ1基因在发育中的内柱中表达。

The amphioxus FoxQ1 gene is expressed in the developing endostyle.

作者信息

Mazet Françoise, Luke Graham N, Shimeld Sebastian M

机构信息

School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.

出版信息

Gene Expr Patterns. 2005 Feb;5(3):313-5. doi: 10.1016/j.modgep.2004.10.004.

DOI:10.1016/j.modgep.2004.10.004

PMID:15661636

Abstract

The FoxQ1 genes form a distinct group within the Fox (also known as forkhead) gene family. We have isolated a gene from the amphioxus Branchiostoma floridae that encodes a forkhead domain with high identity to FoxQ1 genes in other chordates. Molecular phylogenetic analysis places AmphiFoxQ1 in a robust grouping with vertebrate FoxQ1 genes and with Ciona intestinalis Ci-FoxQ1. This group is separate from that containing AmphiFoxQ2, which instead groups with other invertebrate Fox genes. The expression of AmphiFoxQ1 was analysed by whole mount in situ hybridisation. The results show that AmphiFoxQ1 expression is confined to the developing endoderm, and specifically marks the endostyle and associated peripharyngeal bands of amphioxus larvae. Ci-FoxQ1 is also expressed in the endostyle, highlighting this as a conserved site of FoxQ1 gene expression in basal chordates.

摘要

FoxQ1基因在Fox（也称为叉头框）基因家族中形成一个独特的组群。我们从佛罗里达文昌鱼（Branchiostoma floridae）中分离出一个基因，该基因编码的叉头框结构域与其他脊索动物的FoxQ1基因具有高度同源性。分子系统发育分析表明，文昌鱼FoxQ1（AmphiFoxQ1）与脊椎动物的FoxQ1基因以及玻璃海鞘（Ciona intestinalis）的Ci-FoxQ1基因形成一个稳定的类群。该类群与包含文昌鱼FoxQ2（AmphiFoxQ2）的类群不同，后者与其他无脊椎动物的Fox基因归为一类。通过整体原位杂交分析了AmphiFoxQ1的表达情况。结果表明，AmphiFoxQ1的表达局限于发育中的内胚层，并且特异性地标记文昌鱼幼虫的内柱及相关的咽周带。Ci-FoxQ1也在内柱中表达，这突出表明内柱是基础脊索动物中FoxQ1基因表达的一个保守位点。