Yang Hui, Chen Zhong-Ke, Zhang Xiao-Hui, Huang Xiang-Wei, Zhang Pei-Jun, Zhang Hong-Wei
School of life science, Shandong University, Jinan 250100, China.
Cell Biol Int. 2005 Jul;29(7):593-7. doi: 10.1016/j.cellbi.2005.03.012.
A full-length Cks1 homologue gene, AmphiCks1, was identified in amphioxus, Branchiostoma belcheri tsingtauense. Sequence characteristics, phylogeny and patterns of expression during embryonic and larval development were established. The protein predicted from AmphiCks1 showed high sequence identity with vertebrate and invertebrate homologues. Protein structural studies and phylogenetic analysis suggested that Cks homologues are evolutionarily conserved. The AmphiCks1 transcript was detected in most early developmental stages by northern blotting and whole-mount in situ hybridization, suggesting a role for the gene in cell division.
在青岛文昌鱼（Branchiostoma belcheri tsingtauense）中鉴定出了一个全长的Cks1同源基因，即AmphiCks1。确定了其序列特征、系统发育以及在胚胎和幼体发育过程中的表达模式。从AmphiCks1预测的蛋白质与脊椎动物和无脊椎动物的同源物具有高度的序列同一性。蛋白质结构研究和系统发育分析表明，Cks同源物在进化上是保守的。通过Northern印迹和全胚胎原位杂交在大多数早期发育阶段检测到了AmphiCks1转录本，表明该基因在细胞分裂中发挥作用。
