Romero L Michael, Reed J Michael
Department of Biology, Tufts University, Medford, MA 02155, USA.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Jan;140(1):73-9. doi: 10.1016/j.cbpb.2004.11.004.
Evaluating corticosterone (CORT) responses to stress in free-living vertebrates requires knowing the unstressed titers prior to capture. Based upon laboratory data, the assumption has been that samples collected in less than 3 min of capture will reflect these unstressed concentrations. This assumption was tested for six species using samples collected from 945 individuals at 0-6 min after capture. Samples were from five avian species trapped at multiple times of year and one reptilian species, comprising a total of 14 different data sets for comparisons. For seven of 14 data sets, including five species, there was no significant increase in corticosterone titers within 3 min of capture. In six of the 14 data sets, corticosterone titers increased significantly after 2 min, and in one data set, the increase started at 1.5 min. In all seven of the cases showing an increase before 3 min, however, corticosterone titers from the time of increase to 3 min were significantly lower than titers after 30 min of restraint stress. These results indicate a high degree of confidence for these species that samples collected in less than 2 min reflect unstressed (baseline) concentrations, and that samples collected from 2-3 min also will likely reflect baseline concentrations but at worst are near baseline.
评估自由生活的脊椎动物对应激的皮质酮（CORT）反应需要在捕获前了解其非应激水平。基于实验室数据，一直以来的假设是在捕获后不到3分钟内采集的样本将反映这些非应激浓度。使用从945只个体捕获后0至6分钟采集的样本对六个物种进行了该假设的测试。样本来自五个一年中多次捕获的鸟类物种和一个爬行动物物种，总共14个不同的数据集用于比较。在14个数据集中，有7个（包括5个物种）在捕获后3分钟内皮质酮水平没有显著升高。在14个数据集中，有6个在2分钟后皮质酮水平显著升高，在1个数据集中，升高从1.5分钟开始。然而，在所有7个显示在3分钟前升高的案例中，从升高时到3分钟的皮质酮水平显著低于束缚应激30分钟后的水平。这些结果表明，对于这些物种，高度确信在不到2分钟内采集的样本反映非应激（基线）浓度，并且在2至3分钟采集的样本也可能反映基线浓度，但最坏的情况是接近基线。
