Zhao Rui, Luo Jia, Shangguan Dihua, Liu Guoquan
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun, 100080 Beijing, P.R China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):175-81. doi: 10.1016/j.jchromb.2004.11.030.
Viscose fiber, a regenerated cellulose, was evaluated for using as a novel matrix for high performance affinity chromatography. With a one-step activation with epichlorohydrin, heparin can be readily covalently attached to the matrix. This heparin-viscose fiber material was used for purifying antithrombin III (AT III) from human plasma. The purity of the AT III from this one-step purification is 93% as measured by SDS-PAGE and the protein recovery yield is about 90%. This column is highly specific as described by the dissociation constant of the complex of immobilized heparin and AT III, which was 2.83 x 10(-5)mol/L. And more important, this viscose fiber material demonstrated its excellent mechanical property that allows the flow rate to reach up to 900 cm/h or more.
粘胶纤维，一种再生纤维素，被评估用作高性能亲和色谱的新型基质。通过用环氧氯丙烷一步活化，肝素可以很容易地共价连接到基质上。这种肝素-粘胶纤维材料用于从人血浆中纯化抗凝血酶III（AT III）。通过SDS-PAGE测定，该一步纯化得到的AT III纯度为93%，蛋白质回收率约为90%。如固定化肝素与AT III复合物的解离常数所示，该柱具有高度特异性，为2.83×10(-5)mol/L。更重要的是，这种粘胶纤维材料表现出优异的机械性能，允许流速达到900 cm/h或更高。
