Küçük Aysel, Kadioğlu Yücel, Celebi Fikret
Department of Chemistry, Faculty of Science and Arts, Ataturk University, 25240 Erzurum, Turkey.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):203-8. doi: 10.1016/j.jchromb.2004.11.031.
An HPLC system using a new, simple and rapid liquid-liquid extraction and high-performance liquid chromatography-diode array detector method (HPLC-DAD) detection was validated to determine tramadol concentration in rabbit plasma. The method described was applied to a pharmacokinetic study of intravenous tramadol injections in rabbits. The extraction with ethylacetate yielded good response. The recovery of tramadol from plasma averaged 90.40%. Serial plasma samples were obtained prior to, during and after completion of the infusion for determination of tramadol concentrations. Tramadol concentrations were measured using reverse-phase high-performance liquid chromatography and pharmacokinetic application with intravenous tramadol in rabbits revealed that tramadol followed one-compartment open model. Maximum plasma concentration (C(max)) and area under the plasma concentration-time curve (AUC) for tramadol were 14.3 microg mL(-1) and 42.2 microg h mL(-1), respectively. The method developed was successfully applied to a simple, rapid, specific, sensitive and accurate HPLC method for investigation of the pharmacokinetics of tramadol in rabbit plasma.
一种采用新型、简单快速液液萃取及高效液相色谱-二极管阵列检测器法（HPLC-DAD）检测的高效液相色谱系统经验证可用于测定兔血浆中曲马多的浓度。所描述的方法应用于兔静脉注射曲马多的药代动力学研究。用乙酸乙酯萃取得到良好响应。曲马多从血浆中的回收率平均为90.40%。在输注前、输注期间和输注完成后采集系列血浆样本以测定曲马多浓度。采用反相高效液相色谱法测定曲马多浓度，兔静脉注射曲马多的药代动力学应用表明曲马多遵循一室开放模型。曲马多的最大血浆浓度（C(max)）和血浆浓度-时间曲线下面积（AUC）分别为14.3μg mL(-1)和42.2μg h mL(-1)。所建立的方法成功应用于一种简单、快速、特异、灵敏且准确的HPLC方法，用于研究曲马多在兔血浆中的药代动力学。
