Riches James, Morton Ian, Read Robert W, Black Robin M
Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 OJQ, UK.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):251-8. doi: 10.1016/j.jchromb.2004.11.039.
A sensitive method has been developed for the trace analysis of alkyl alkylphosphonic acids, metabolites of nerve agents, in urine using a benchtop ion trap mass spectrometer. The acids were isolated from urine by simple solid phase extraction and converted to their pentafluorobenzyl esters. An ion trap mass spectrometer in selected reaction monitoring mode provided limits of detection of 0.1 ng/ml for isopropyl, isobutyl, pinacolyl and cyclohexyl methylphosphonic acids and for ethyl ethylphosphonic acid. The detection limit for ethyl methylphosphonic acid was higher (0.5 ng/ml) due to a lower recovery.
已开发出一种灵敏的方法，用于使用台式离子阱质谱仪对尿液中神经毒剂代谢产物烷基烷基膦酸进行痕量分析。通过简单的固相萃取从尿液中分离出这些酸，并将其转化为五氟苄酯。处于选择反应监测模式的离子阱质谱仪对异丙基、异丁基、频哪基和环己基甲基膦酸以及乙基乙基膦酸的检测限为0.1 ng/ml。由于回收率较低，甲基乙基膦酸的检测限较高（0.5 ng/ml）。
