A群链球菌4型和11型T血清型分离株中高水平四环素耐药的分子机制
Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11.
作者信息
Matsumoto Masakado, Sakae Kenji, Ohta Michio, Endo Miyoko, Okuno Rumi, Murayama Shoko, Hirasawa Kyoko, Suzuki Rieko, Isobe Junko, Tanaka Daisuke, Katsukawa Chihiro, Tamaru Aki, Tomita Masaaki, Ogata Kikuyo, Yasuoka Tomihisa, Ikebe Tadayoshi, Watanabe Haruo
机构信息
Department of Microbiology, Aichi Prefectural Institute of Public Health, Nagoya 462-8576, Japan.
出版信息
Int J Antimicrob Agents. 2005 Feb;25(2):142-7. doi: 10.1016/j.ijantimicag.2004.09.014.
The molecular mechanism of high level tetracycline resistance in T serotypes 4 and 11 group A streptococcal (GAS) isolates was examined in 61 tetracycline-resistant isolates in Japan. PCR and sequencing analyses revealed that the T serotype/emm genotype, T4/4 isolates carried tet(O) genes, which were genetically homogenous. The T11/11 and T11/89 isolates carried different subtypes of tet(M) genes, which were present on transposons Tn916 and Tn1545, respectively. In addition, these T11 isolates may have obtained the tet(M) gene after the 1990s, because resistance to tetracycline in T11 isolates was rarely found before then. These results strongly suggested that the T4 and T11 GAS isolates acquired tetracycline-resistance via different molecular mechanisms.
在日本的61株四环素耐药性A群链球菌（GAS）分离株中，研究了T血清型4和11型高水平四环素耐药性的分子机制。PCR和测序分析表明，T血清型/ emm基因型中，T4/4分离株携带tet(O)基因，这些基因在遗传上是同质的。T11/11和T11/89分离株分别携带tet(M)基因的不同亚型，这些亚型分别存在于转座子Tn916和Tn1545上。此外，这些T11分离株可能在20世纪90年代后获得了tet(M)基因，因为在那之前很少发现T11分离株对四环素耐药。这些结果有力地表明，T4和T11 GAS分离株通过不同的分子机制获得了四环素耐药性。
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