通过引入顺式元件重复序列对米曲霉烯醇化酶启动子（P-enoA）进行改进。
Improvement of the Aspergillus oryzae enolase promoter (P-enoA) by the introduction of cis-element repeats.
作者信息
Tsuboi Hirokazu, Koda Akio, Toda Tomomi, Minetoki Toshitaka, Hirotsune Masato, Machida Masayuki
机构信息
General Research Laboratory, Ozeki Corporation, Hyogo, Japan.
出版信息
Biosci Biotechnol Biochem. 2005 Jan;69(1):206-8. doi: 10.1271/bbb.69.206.
We constructed a protein expression vector with an improved enoA promoter that harbored 12 tandem repeats of the cis-acting element (region III) of Aspergillus oryzae. The improved promoter yielded reporter beta-glucuronidase (GUS) activity approximately 30-fold of the original promoter. Northern blot analysis confirmed that GUS expression was increased at the transcriptional level. The transformant harboring seven copies of the novel vector showed more than 100,000 U/mg GUS protein, which was approximately 30% of all the cell-free soluble proteins.
我们构建了一个带有改进型enoA启动子的蛋白质表达载体，该启动子含有米曲霉顺式作用元件（区域III）的12个串联重复序列。改进后的启动子产生的报告基因β-葡萄糖醛酸酶（GUS）活性约为原始启动子的30倍。Northern印迹分析证实GUS表达在转录水平上有所增加。携带七个新型载体拷贝的转化体显示出超过100,000 U/mg的GUS蛋白，约占所有无细胞可溶性蛋白的30%。
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